Assessment of microbial diversity and enumeration of metal tolerant autochthonous bacteria from tailings of magnesite and bauxite mines

Narayanan, Mathiyazhagan; Devarajan, Natarajan; He, Zhixia; Kandasamy, Sabariswaran; Ashokkumar, Veeramuthu; Rathinam, Raja; Carvalho, Isabel S.

http://hdl.handle.net/10400.1/16619

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Authors

Narayanan, Mathiyazhagan

Devarajan, Natarajan

He, Zhixia

Kandasamy, Sabariswaran

Ashokkumar, Veeramuthu

Rathinam, Raja

Carvalho, Isabel S.

Abstract(s)

The magnesite and bauxite mines are a major source for some minerals and metals. The tailings of these magnesite and bauxite mines contains massive amount of Mn. (3221.2 +/- 6.51 & 7102.8.4 +/- 10.24), Cd (40.95 +/- 2.12 & 892.86 +/- 8.48), Zn (951.08 +/- 4.31 &724.12 +/- 3.18), and Pb (425.8 +/- 5.21 & 812.13 +/- 9.19 mg Kg(-1)) with alkaline and acid pH respectively. In the microbial diversity analysis, the results 8 (Thiobacillus thiooxidans, Leptospirillum ferrooxidans, Acetobacter methanolicus, T. intermedius, Bacillus cereus, Sulfobacillus acidophilus, Methylobacterium sp, and Thiobacillus ferooxidans) and 12 (Serratia marcescens, Metalogenium symbioticum 1, Metallogenium symbioticum 2, Bacillus alcalophilus, Aminobacter sp, Naumaniella neustonica, Staphylococcus aureus 1, Methylbacillus sp, Pandoraea sputorum, Acenetobacter sp, S. aureus 2, Pseudomonas aeruginosa) bacteria species were isolated from bauxite and magnesite mine tailings. The fungus such as Rhizopus arrhizus, Mucor sp, Aspergillus niger and Penicillium sp were obtained in both acid and alkaline environment. Among this microbial diversity, P. sputorum and B. cereus (identified through 16S rDNA sequencing) were shown better resistance to these four metals for up to 250 mg L-1. The bacterial diversity indexes, concludes that there was least diverse among the three sites. The evenness/equitability index also conform lower level of variation among these sites. (C) 2019 Elsevier Ltd. All rights reserved.