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Ultrasonic-assisted extraction and natural deep eutectic solvents combination: A green strategy to improve the recovery of phenolic compounds from Lavandula Pedunculata subsp. Lusitanica (Chaytor) Franco
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Advisor(s)
Abstract(s)
The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp.
lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used
in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE)
for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline
chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw)
and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-
picrylhydrazyl (DPPH) and 2.20
-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods,
respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and
AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts
with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified
by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds.
The results confirmed that the combination of UAE and NADES provide an excellent alternative to
organic solvents for sustainable and green extraction, and have huge potential for use in industrial
applications involving the extraction of bioactive compounds from plants.
Description
Keywords
Natural deep eutectic solvents (NADES) Ultrasound-assisted extraction (UAE) Lavandula pedunculata Phenolic compounds Phenolic acids Hydroxycinnamic acids Antioxidant activity Enzyme inhibition HPLC Mass spectrometry
Citation
Antioxidants 10 (4): 582 (2021)
Publisher
MDPI