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Aqueous extracts from tunisian diplotaxis: phenol content, antioxidant and anti-acetylcholinesterase activities, and impact of exposure to simulated gastrointestinal fluids

2016-07Journal article Open access
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dc.contributor.authorBahloul, Nada
dc.contributor.authorBellili, Sana
dc.contributor.authorAAZZA, Smail
dc.contributor.authorCherif, Ameur
dc.contributor.authorFaleiro, Leonor
dc.contributor.authorAntunes, Maria Dulce
dc.contributor.authorMiguel, Maria Graça
dc.contributor.authorMnif, Wissem
dc.date.accessioned2017-04-07T15:56:33Z
dc.date.available2017-04-07T15:56:33Z
dc.date.issued2016-07
dc.description.abstractAntioxidants have been considered essential for preventing cell damage by scavenging deleterious free radicals. The consumption of antioxidant-rich plants is associated with a reduced risk of some chronic diseases. This study evaluates the antioxidant and acetylcholinesterase inhibition activities of aqueous extracts obtained from different parts of Diplotaxis simplex and Diplotaxis harra from Tunisia. The study also aimed to investigate the action of simulated gastrointestinal juice on antioxidant activities of both extracts. The total phenolic, flavone and flavonol, and flavanone and dihydroflavonol contents were determined by Folin-Ciocalteau, aluminum chloride and 2,4-dinitrophenylhydrazine colorimetric methods, respectively. The metal ion chelating activity, acetylcholinesterase inhibition capacity, and free radical scavenging potential of the extracts towards ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), hydroxyl, superoxide and nitric oxide were also evaluated. The action of simulated gastro-intestinal fluids on the flavone and flavonol content and total antioxidant activity of the flower extracts was surveyed. Extracts from the seeds and flowers of D. simplex and D. harra displayed the highest amounts of phenols (2691.7 and 2694.5 mg Caffeic Acid Equivalent (CAE)/100 mg; 3433.4 and 2647.2 mg CAE/100 mg, respectively) and flavonols/flavones (2144.4 and 2061.1 mg Rutin Equivalent (RE)/100 g; 1922.6 and 1461.1 mg RE/100 g, respectively). The flower and seed extracts exhibited the highest rates of antioxidant and acetylcholinesterase inhibition activities. A decrease in the flavonoid content and antioxidant activity was observed after extract exposure to simulated saliva. Antioxidant and acetylcholinesterase inhibition activities were noted to depend on plant species and plant parts. In vitro gastrointestinal digestion is useful in assessing the bio-accessibility of compounds with biological activities from food. The simulated gastrointestinal fluids influenced the flavonoid concentration and antioxidant activity.
dc.identifier.doi10.3390/antiox5020012
dc.identifier.issn2076-3921
dc.identifier.otherAUT: MFA00238; MGM00322;
dc.identifier.urihttp://hdl.handle.net/10400.1/9451
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relation.isbasedonWOS:000380035400003
dc.titleAqueous extracts from tunisian diplotaxis: phenol content, antioxidant and anti-acetylcholinesterase activities, and impact of exposure to simulated gastrointestinal fluids
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.citation.endPage12
oaire.citation.issue2
oaire.citation.startPage12
oaire.citation.titleAntioxidants
oaire.citation.volume5
oaire.fundingStream3599-PPCDT
person.familyNameAAZZA
person.familyNameFaleiro
person.familyNameAntunes
person.familyNameMiguel
person.givenNameSmail
person.givenNameMaria Leonor
person.givenNameMaria Dulce
person.givenNameMaria da Graça
person.identifier1426445
person.identifier252666
person.identifier177556
person.identifier.ciencia-idE416-77DF-6A97
person.identifier.ciencia-id281D-5E01-F09A
person.identifier.ciencia-idC11B-9B05-217E
person.identifier.ciencia-id6110-6FE7-DF28
person.identifier.orcid0000-0002-9339-8999
person.identifier.orcid0000-0002-3878-6948
person.identifier.orcid0000-0002-8913-6136
person.identifier.orcid0000-0003-2507-4228
person.identifier.ridL-7630-2017
person.identifier.ridA-4683-2012
person.identifier.scopus-author-id51664684500
person.identifier.scopus-author-id6507927018
person.identifier.scopus-author-id7102645075
person.identifier.scopus-author-id35562054500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication5361e29a-3b79-4ce8-9f87-0c667d60588d
relation.isAuthorOfPublication7947cc50-4ae0-4ada-8ddf-081f247adc90
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relation.isAuthorOfPublication.latestForDiscovery7947cc50-4ae0-4ada-8ddf-081f247adc90
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