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DNA damage and lipid peroxidation vs. protection responses in the gill of Dicentrarchus labrax L. from a contaminated coastal lagoon (Ria de Aveiro, Portugal)

2008Journal article Restricted access
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dc.contributor.authorAhmad, I.
dc.contributor.authorMaria, Vera L.
dc.contributor.authorOliveira, M.
dc.contributor.authorSerafim, Angela
dc.contributor.authorBebianno, Maria João
dc.contributor.authorPacheco, M.
dc.contributor.authorSantos, M.A.
dc.date.accessioned2020-05-05T18:05:16Z
dc.date.available2020-05-05T18:05:16Z
dc.date.issued2008
dc.description.abstractThe present research work aimed to investigate the damage vs. protection responses in gill of European sea bass (Dicentrarchus labrax) captured at a polluted coastal lagoon, Ria de Aveiro (Portugal), as a tool to evaluate the human impacts on environmental health. Damage was assessed as DNA strand breakage and lipid peroxidation (LPO) whereas protection was evaluated by measuring catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione S-transferase (GST), total glutathione (GSHt), thiols and metallothioneins (MT). Fish were caught at five locations: Gafanha (GAF), Rio Novo do Príncipe (RIO), Laranjo (LAR) and Vagos (VAG) presenting each a different recognized source of contamination, and Torreira (TOR), assumed as reference site. Among the surveyed sites, gill damage was observed only at GAF, as measured by DNA integrity loss and LPO increase. An overall induction in enzymatic antioxidant protection was perceptible in fish from GAF and VAG, expressed as higher GPX, GR and GST activities. In addition, LAR fish showed elevated GST and CAT activities. Thiols content was higher in all study sites irrespective to the pollution spectrum, whereas GSHt increase was only observed at GAF and VAG. The highest MT level was detected in fish from VAG and the lowest level from RIO suggesting a low contamination degree on this particular site. Globally, the results expressed site-specific response patterns, signalling two critical areas - GAF and VAG. Additionally, a joint analysis (damage vs. protection) allowed the following ordering of surveyed sites according to the contamination degree and risk to fish health; GAF>VAG>LAR>RIO>TOR. D. labrax gill responses demonstrated their efficacy as early warning signals of the contaminants presence. Moreover, the adopted approach, considering simultaneously protection responses and damaging effects, also revealed its usefulness on the pollution extent assessment.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technology - SFRH/BPD/34326/ 2006pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1016/j.scitotenv.2008.06.027pt_PT
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.1/13836
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0048969708006815pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBasspt_PT
dc.subjectDNA damagept_PT
dc.subjectGeographypt_PT
dc.subjectGillspt_PT
dc.subjectLipid peroxidationpt_PT
dc.subjectPortugalpt_PT
dc.subjectWater Pollutantspt_PT
dc.subjectEnvironmental monitoringpt_PT
dc.subjectWetlandspt_PT
dc.subjectChemicalpt_PT
dc.subjectDicentrarchus labrax L.pt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectOxidative stresspt_PT
dc.subjectMetallothioneinpt_PT
dc.subjectDamage vs. protectionpt_PT
dc.subjectRia de Aveiropt_PT
dc.titleDNA damage and lipid peroxidation vs. protection responses in the gill of Dicentrarchus labrax L. from a contaminated coastal lagoon (Ria de Aveiro, Portugal)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F26970%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F27584%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FMAR%2F61195%2F2004/PT
oaire.citation.endPage307pt_PT
oaire.citation.issue1-2pt_PT
oaire.citation.startPage298pt_PT
oaire.citation.titleScience of the Total Environmentpt_PT
oaire.citation.volume406pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStreamPOCI
person.familyNameMaria
person.familyNamePereira Serafim
person.familyNameBebianno
person.givenNameVera Lúcia
person.givenNameMaria Angela
person.givenNameMaria
person.identifier.ciencia-id7C1D-5627-D3BC
person.identifier.ciencia-id9C18-090A-5E3D
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0002-0331-3489
person.identifier.orcid0000-0002-1165-0645
person.identifier.orcid0000-0003-1492-8566
person.identifier.ridI-5142-2013
person.identifier.scopus-author-id7004664869
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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