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New insights on vitamin K metabolism in senegalese sole (Solea senegalensis) based on ontogenetic and tissue-specific vitamin K epoxide reductase molecular data

2020Journal article Open access
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dc.contributor.authorBeato, Silvia
dc.contributor.authorMarques, Carlos
dc.contributor.authorLaizé, Vincent
dc.contributor.authorGavaia, Paulo
dc.contributor.authorFernández, Ignacio
dc.date.accessioned2020-09-03T09:13:41Z
dc.date.available2020-09-03T09:13:41Z
dc.date.issued2020
dc.description.abstractVitamin K (VK) is a key nutrient for several biological processes (e.g., blood clotting and bone metabolism). To fulfill VK nutritional requirements, VK action as an activator of pregnane X receptor (Pxr) signaling pathway, and as a co-factor of γ-glutamyl carboxylase enzyme, should be considered. In this regard, VK recycling through vitamin K epoxide reductases (Vkors) is essential and should be better understood. Here, the expression patterns of vitamin K epoxide reductase complex subunit 1 (vkorc1) and vkorc1 like 1 (vkorc1l1) were determined during the larval ontogeny of Senegalese sole (Solea senegalensis), and in early juveniles cultured under different physiological conditions. Full-length transcripts for ssvkorc1 and ssvkorc1l1 were determined and peptide sequences were found to be evolutionarily conserved. During larval development, expression of ssvkorc1 showed a slight increase during absence or low feed intake. Expression of ssvkorc1l1 continuously decreased until 24 h post-fertilization, and remained constant afterwards. Both ssvkors were ubiquitously expressed in adult tissues, and highest expression was found in liver for ssvkorc1, and ovary and brain for ssvkorc1l1. Expression of ssvkorc1 and ssvkorc1l1 was differentially regulated under physiological conditions related to fasting and re-feeding, but also under VK dietary supplementation and induced deficiency. The present work provides new and basic molecular clues evidencing how VK metabolism in marine fish is sensitive to nutritional and environmental conditions.pt_PT
dc.description.sponsorshipFCT: UIDB/04326/2020. ALGASOLE-16-02-01-FMP-0058; RTI2018-099029-A-I00; RYC2018-025337-I.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms21103489pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.1/14675
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectVitamin Kpt_PT
dc.subjectGene expressionpt_PT
dc.subjectEvolutionpt_PT
dc.subjectFlatfishpt_PT
dc.subjectNutritionpt_PT
dc.subjectPollutionpt_PT
dc.titleNew insights on vitamin K metabolism in senegalese sole (Solea senegalensis) based on ontogenetic and tissue-specific vitamin K epoxide reductase molecular datapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F82049%2F2011/PT
oaire.citation.issue10pt_PT
oaire.citation.startPage3489pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume21pt_PT
person.familyNameLaizé
person.familyNameGavaia
person.givenNameVincent
person.givenNamePaulo
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0002-9582-1957
person.identifier.ridB-4463-2008
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
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relation.isProjectOfPublicationc92313d2-71c0-4b43-9593-b689bf4396a3
relation.isProjectOfPublication.latestForDiscoveryc92313d2-71c0-4b43-9593-b689bf4396a3

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