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Evidence that 1-methyl-l-tryptophan is a food-related odorant for the Senegalese sole (Solea senegalensis)

2011-04Journal article Restricted
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dc.contributor.authorVelez, Zélia
dc.contributor.authorHubbard, Peter
dc.contributor.authorHardege, Jörg D.
dc.contributor.authorWelham, K.
dc.contributor.authorBarata, E. N.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-09T10:35:20Z
dc.date.available2014-05-09T10:35:20Z
dc.date.issued2011-04
dc.date.updated2014-05-08T10:04:08Z
dc.description.abstractNocturnal animals often rely heavily on olfactory cues to locate their food. This is especially true for fish whose prey live buried in the substrate. The aim of the current study was to identify non-polar odorants released by the ragworm (Hediste diversicolor) that are detected by the olfactory system of the Senegalese sole (Solea senegalensis) and therefore may be food-related cues. Ragworm-conditioned water was passed through a C18 solid-phase extraction cartridge (to remove polar compounds) and the eluate fractionated by reverse phase high-performance liquid chromatography (HPLC). The fractions were then tested for olfactory activity in the sole by the electro-olfactogram (EOG). Most olfactory activity was found in the first two fractions to elute from the HPLC column (0–4 min). Liquid chromatography-mass spectrometry (LC/MS) of both fractions revealed a base peak with a molecular mass of 219.4 Da and a fragment of 205.3 Da. These data are consistent with a methylated form of tryptophan; synthetic 1-methyl-l-tryptophan had a similar HPLC retention time (2.8 min) and similar LC/MS spectra. Furthermore, sole showed olfactory sensitivity to 1-methyl-l-tryptophan with the lower (left) epithelium being more sensitive than the upper (right). Cross-adaptation (using EOG recording) suggested that the olfactory receptors responding to 1-methyl-l-tryptophan are different in the lower epithelium from the upper. These results suggest that ragworms release 1-methyl-l-tryptophan, or similar tryptophan derivative, and that sole may use this chemical cue to locate and/or identify one of their main prey species. This may help the formation of artificial feeds that are more attractive to sole.por
dc.identifier.citationVelez, Zélia; Hubbard, Peter C.; Hardege, Jörg D.; Welham, Kevin J.; Barata, Eduardo N.; Canário, Adelino V.M.Evidence that 1-methyl-l-tryptophan is a food-related odorant for the Senegalese sole (Solea senegalensis), Aquaculture, 314, 1-4, 153-158, 2011.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquaculture.2011.01.025
dc.identifier.issn0044-8486
dc.identifier.otherAUT: ACA00258
dc.identifier.urihttp://hdl.handle.net/10400.1/3822
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0044848611000652por
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectOlfactionpor
dc.subjectRagwormpor
dc.subjectFeedingpor
dc.subjectAsymmetrypor
dc.subjectPredatorpor
dc.subjectPreypor
dc.subjectFlatfishpor
dc.titleEvidence that 1-methyl-l-tryptophan is a food-related odorant for the Senegalese sole (Solea senegalensis)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage158por
oaire.citation.issue1-4por
oaire.citation.startPage153por
oaire.citation.titleAquaculturepor
oaire.citation.volume314por
person.familyNameVelez
person.familyNameHubbard
person.familyNameBarata
person.familyNameCanario
person.givenNameZélia
person.givenNamePeter
person.givenNameEduardo Nuno
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id4412-F95B-34A2
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-2761-4048
person.identifier.orcid0000-0002-3007-4647
person.identifier.orcid0000-0001-9714-5967
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-5283-2013
person.identifier.ridB-2823-2008
person.identifier.ridB-2902-2010
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id16176444400
person.identifier.scopus-author-id7005928467
person.identifier.scopus-author-id6602073256
person.identifier.scopus-author-id56568523700
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3292b0fd-8d08-4512-b512-a04b31a7926a
relation.isAuthorOfPublicationf599b3ac-806b-4f65-9eb6-f70e68bcb142
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relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
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