Repository logo
 
Publication

Development of a new eco-friendly ultrasound-assisted extraction method to quantify tryptophan in wild mushrooms and determination of its beneficial properties

2025-02Journal article Open access
Simple item page
dc.contributor.authorLópez, Alejandro R.
dc.contributor.authorOrtega-Caneda, Elena
dc.contributor.authorEspada-Bellido, Estrella
dc.contributor.authorChinchilla, Nuria
dc.contributor.authorPalma, Miguel
dc.contributor.authorAliaño González, María José
dc.contributor.authorBarbero, Gerardo Fernández
dc.contributor.authorCarrera, Ceferino
dc.date.accessioned2024-12-12T12:24:51Z
dc.date.available2024-12-12T12:24:51Z
dc.date.issued2025-02
dc.description.abstractBioactive compounds in food offer health benefits by influencing cellular and physiological functions. Tryptophan, an essential amino acid and precursor to neurotransmitters like melatonin and serotonin, regulates mood and circadian rhythms. However, its quantification in mushrooms is scarce studied. An eco-friendly ultrasound- assisted extraction (UAE) method has been optimized to quantify tryptophan using ultra high-performance liquid chromatography with a diode array and fluorescence detector (UHPLC-DAD-Fl). Tryptophan levels were determined in 26 wild mushroom samples of the genus Lactarius and Boletus. The concentrations ranged from 0.042 mg/g to 0.742 mg/g. The extracts' health benefits were assessed for antioxidant capacity using DPPH (17.7 %71.6 %) and ABTS (7.2 %24.9 %) methods, while acetylcholinesterase (AChE) inhibitory activity ranged from 13.1 % to 49.8 %. Promising results were obtained. Hierarchical cluster analysis demonstrated a correlation between tryptophan concentration, mushroom species, location, and extract properties, highlighting tryptophan's crucial role in these mushrooms' health benefits.eng
dc.description.sponsorshipUCA/R62REC/2021; UCA/R96REC/2018; 001ENE2019; 002ENE2019
dc.identifier.doi10.1016/j.foodchem.2024.142006
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10400.1/26459
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relation.ispartofFood Chemistry
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBioactive compounds
dc.subjectTryptophan
dc.subjectMushrooms
dc.subjectUltrasound-assisted extraction
dc.subjectBox-Behnken design
dc.subjectAcetylcholinesterase
dc.subjectAntioxidant capacity
dc.titleDevelopment of a new eco-friendly ultrasound-assisted extraction method to quantify tryptophan in wild mushrooms and determination of its beneficial propertieseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage142006
oaire.citation.titleFood Chemistry
oaire.citation.volume465
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAliaño González
person.givenNameMaría José
person.identifier456042
person.identifier.ciencia-idDD17-3640-DB88
person.identifier.orcid0000-0002-5816-4476
person.identifier.scopus-author-id57200221253
relation.isAuthorOfPublication305fc84f-d79e-49d4-ba14-bea107dcc203
relation.isAuthorOfPublication.latestForDiscovery305fc84f-d79e-49d4-ba14-bea107dcc203

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1-s2.0-S0308814624036562-main.pdf
Size:
3.53 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)