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Ultrasensitive gold micro-structured electrodes enabling the detection of extra-cellular long-lasting potentials in astrocytes populations

2017-10Journal article Open access
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dc.contributor.authorMestre, Ana L. G.
dc.contributor.authorCerquido, Monica
dc.contributor.authorINÁCIO, PEDRO
dc.contributor.authorAsgarifar, Sanaz
dc.contributor.authorLourenco, Ana S.
dc.contributor.authorLurdes S. Cristiano, M.
dc.contributor.authorAguiar, Paulo
dc.contributor.authorMedeiros, Maria C. R.
dc.contributor.authorAraújo, Inês
dc.contributor.authorVentura, Joao
dc.contributor.authorGomes, Henrique L.
dc.date.accessioned2018-12-07T14:58:08Z
dc.date.available2018-12-07T14:58:08Z
dc.date.issued2017-10
dc.description.abstractUltra-sensitive electrodes for extracellular recordings were fabricated and electrically characterized. A signal detection limit defined by a noise level of 0.3-0.4 mu V for a bandwidth of 12.5 Hz was achieved. To obtain this high sensitivity, large area (4 mm(2)) electrodes were used. The electrode surface is also micro-structured with an array of gold mushroom-like shapes to further enhance the active area. In comparison with a flat gold surface, the micro-structured surface increases the capacitance of the electrode/electrolyte interface by 54%. The electrode low impedance and low noise enable the detection of weak and low frequency quasi-periodic signals produced by astrocytes populations that thus far had remained inaccessible using conventional extracellular electrodes. Signals with 5 mu V in amplitude and lasting for 5-10 s were measured, with a peak-to-peak signal-to-noise ratio of 16. The electrodes and the methodology developed here can be used as an ultrasensitive electrophysiological tool to reveal the synchronization dynamics of ultra-slow ionic signalling between non-electrogenic cells.
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT), through the project "Implantable organic devices for advanced therapies" (INNOVATE) [PTDC/EEI-AUT/5442/2014]; Instituto de Telecomunicacoes [UID/Multi/04326/2013]; Associated Laboratory - Institute of Nanoscience and Nanotechnology [POCI-01-0145-FEDER-016623]; [PTDC/CTM-NAN/3146/2014]
dc.identifier.doi10.1038/s41598-017-14697-y
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/11881
dc.language.isoeng
dc.peerreviewedyes
dc.publisherNature Publishing Group
dc.subjectCalcium waves
dc.subjectIn-Situ
dc.subjectImpedance spectroscopy
dc.subjectMicroelectrode arrays
dc.subjectConducting polymers
dc.subjectNeural interfaces
dc.subjectOscillations
dc.subjectNetworks
dc.subjectCulture
dc.subjectNoise
dc.titleUltrasensitive gold micro-structured electrodes enabling the detection of extra-cellular long-lasting potentials in astrocytes populations
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage14284
oaire.citation.titleScientific Reports
oaire.citation.volume7
person.familyNameINÁCIO
person.familyNameAsgarifar
person.familyNameLourenço
person.familyNameCristiano
person.familyNamePombinho de Araújo
person.familyNameGomes
person.givenNamePEDRO
person.givenNameSanaz
person.givenNameAna Sofia
person.givenNameMaria de Lurdes
person.givenNameInês Maria
person.givenNameHenrique Leonel
person.identifier2001885
person.identifierhttps://scholar.google.com/citations?hl=en&user=LFpifG0AAAAJ
person.identifier170713
person.identifierF-4703-2012
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person.identifier.ciencia-idE411-6006-5A01
person.identifier.ciencia-idD11F-D4CA-2947
person.identifier.orcid0000-0003-1986-7918
person.identifier.orcid0000-0002-8724-0044
person.identifier.orcid0000-0003-2882-6026
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person.identifier.orcid0000-0002-2438-0111
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person.identifier.ridG-2345-2012
person.identifier.scopus-author-id55938897300
person.identifier.scopus-author-id24823836800
person.identifier.scopus-author-id56822397300
person.identifier.scopus-author-id9238724800
person.identifier.scopus-author-id56271084100
person.identifier.scopus-author-id7005305880
rcaap.rightsopenAccess
rcaap.typearticle
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