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Analysis of the transthyretin-like (TTL) gene family in Ostertagia ostertagi - comparison with other strongylid nematodes and Caenorhabditis elegans

2008Journal article Restricted access
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dc.contributor.authorSaverwyns, H.
dc.contributor.authorVisser, A.
dc.contributor.authorVan Durme, J.
dc.contributor.authorPower, Deborah
dc.contributor.authorMorgado, Isabel
dc.contributor.authorKennedy, M. W.
dc.contributor.authorKnox, D. P.
dc.contributor.authorSchymkowitz, J.
dc.contributor.authorRousseau, F.
dc.contributor.authorGevaert, K.
dc.contributor.authorVercruysse, J.
dc.contributor.authorClaerebout, E.
dc.contributor.authorGeldhof, P.
dc.date.accessioned2014-10-15T13:23:13Z
dc.date.available2014-10-15T13:23:13Z
dc.date.issued2008
dc.description.abstractThe transthyretin-like (ttl) gene family is one of the largest conserved nematode-specific gene families, coding for a group of proteins with significant sequence similarity to transthyretins (TTR) and transthyretin-related proteins (TRP). In the present study, we investigated the ttl family in Ostertagia ostertagi (a nematode of the abomasum of cattle). Mining of expressed sequence tag (EST) databases revealed the presence of at least 18 ttl genes in O. ostertagi (Oo-ttl), most of which are constitutively transcribed from the free-living, third larval stage onwards. The full-length cDNA of one of these genes (Oo-ttl-1) was amplified and cloned for recombinant expression. Western blot analysis using a specific antiserum showed that the native protein Oo-TTL-1 was highly present in the excretory–secretory (ES) products of adults of O. ostertagi. The protein was immunolocalized to the pseudocoelomic fluid of adult worms. A phylogenetic–bioinformatic analysis of all amino acid sequence data for TTL proteins from a range of strongylid nematodes showed that they could be divided into at least five different classes. This classification was based on conserved amino acids in the first TTL signature domain and the number and location of cysteine residues. The biological role(s) of the TTLs in nematode biology is still unclear. A theoretical threedimensional model of Oo-TTL-1 indicated that it had a similar structure to TTRs (i.e., containing b-sheets, arranged in a b-sandwich). In contrast to TTRs, competitive binding studies using recombinant Oo-TTL-1 indicated that the protein was devoid of any hydrophobic ligand- or thyroid hormone-binding properties. Finally, combinatorial analysis by double-stranded RNA interference of five ttl genes in the free-living nematode Caenorhabditis elegans did not reveal any visible phenotypes. More information on the transcription profile and tissue distribution of TTLs in nematodes is needed to provide new insights into the biological role of this gene family. 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.por
dc.description.sponsorshipH.S. and A.V. were supported by a grant from Research Foundation-Flanders (F.W.O. – Vlaanderen). P.G. and J.V.D. are Postdoctoral Fellows of the Fund for Scientific Research – Flanders (Belgium) (F.W.O. – Vlaanderen). The authors would like to thank W. Van Den Broeck and L. Debels for their assistance with the tissue sections.por
dc.identifier.citationH. Saverwyns, A. Visser, J. Van Durme, D. Power, I. Morgado, M.W. Kennedy, D.P. Knox, J. Schymkowitz, F. Rousseau, K. Gevaert, J. Vercruysse, E. Claerebout, P. Geldhof, "Analysis of the transthyretin-like (TTL) gene family in Ostertagia ostertagi - comparison with other strongylid nematodes and Caenorhabditis elegans" in International Journal for Parasitology 38 (2008) 1545–1556.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ijpara.2008.04.004
dc.identifier.issn0020-7519
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5323
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectTranthyretin-likepor
dc.subjectOstertagia ostertagipor
dc.subjectNematode-specific moleculespor
dc.titleAnalysis of the transthyretin-like (TTL) gene family in Ostertagia ostertagi - comparison with other strongylid nematodes and Caenorhabditis eleganspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1556por
oaire.citation.startPage1545por
oaire.citation.titleInternational Journal for Parasitologypor
oaire.citation.volume38por
person.familyNamePower
person.familyNameMorgado
person.givenNameDeborah Mary
person.givenNameIsabel
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id3315-7715-C3B4
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-7826-997X
person.identifier.ridJ-4798-2013
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id18134268300
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication67026aa2-ccdb-4b68-9938-b09e0fd6aca0
relation.isAuthorOfPublication.latestForDiscovery67026aa2-ccdb-4b68-9938-b09e0fd6aca0

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