Publication
Pulmonaria mollis: rediscovering a forgotten edible plant through phytochemical profiling and bioactivity assessment
| dc.contributor.author | Srećković, Nikola | |
| dc.contributor.author | Monti, Daria Maria | |
| dc.contributor.author | Liberti, Davide | |
| dc.contributor.author | Mišić, Danijela | |
| dc.contributor.author | Gašić, Uroš | |
| dc.contributor.author | Matić, Sanja Lj | |
| dc.contributor.author | Mihailović, Vladimir | |
| dc.date.accessioned | 2025-09-29T09:43:28Z | |
| dc.date.available | 2025-09-29T09:43:28Z | |
| dc.date.issued | 2025-09 | |
| dc.description.abstract | Pulmonaria species were historically valued as traditional remedies for respiratory ailments and as wild food, yet their culinary and medicinal applications have largely been forgotten. Among them, Pulmonaria mollis remains underinvestigated, with scarce phytochemical and pharmacological data. This study aimed to fill this gap by investigating the phenolic composition and biological activities of methanol extracts from the aerial parts (PMA) and roots (PMR) of P. mollis. UHPLC–MS4 Orbitrap analysis identified 59 compounds, mostly phenolic acids. Quantitative profiling by UHPLC/DAD/(− )HESI-MS/MS showed PMA was rich in rosmarinic acid (4508.27 μg/ g), p-hydroxybenzoic acid (605.70 μg/g), and caffeic acid (325.42 μg/g), whereas PMR contained higher levels of salvianolic acids A (2311.09 μg/g) and B (4697.98 μg/g). Along with significant antioxidant activity confirmed by standard spectrophotometric assays, where PMR showed slightly stronger radical scavenging capacity, both extracts effectively protected DNA from hydroxyl- and peroxyl-induced oxidative damage. In enzyme inhibition assays, both extracts demonstrated α-amylase inhibitory effects, while PMR showed particularly potent α-glucosidase inhibition (IC50 = 14.00 μg/mL), outperforming acarbose. Moderate antibacterial and antifungal activities were also observed, with PMR generally more effective, whereas cytotoxicity testing indicated selective activity against A431 (IC50 values of 178 ± 11 μg/mL and 64 ± 3 μg/mL for PMA and PMR, respectively) cells without affecting normal cells. Overall, the findings highlight P. mollis as a promising candidate for development into functional foods and nutraceuticals aimed at chronic disease prevention, providing valuable phenolic compounds and biological properties that support its potential use as an alternative to the commonly used Pulmonaria officinalis. | eng |
| dc.description.sponsorship | 451–03-136/2025–03/200122; 451-03-137/2025-03/200122; 451-03-136/2025-03/200378; 451-03-136/2025-03/200007 | |
| dc.identifier.doi | 10.1016/j.fbio.2025.107396 | |
| dc.identifier.issn | 2212-4292 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/27749 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation.ispartof | Food Bioscience | |
| dc.rights.uri | N/A | |
| dc.subject | Pulmonaria mollis | |
| dc.subject | Phenolic compounds | |
| dc.subject | Antioxidant activity | |
| dc.subject | Antidiabetic potential | |
| dc.subject | Antimicrobial activity | |
| dc.subject | Antigenotoxicity | |
| dc.subject | Biocompatibility | |
| dc.title | Pulmonaria mollis: rediscovering a forgotten edible plant through phytochemical profiling and bioactivity assessment | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 107396 | |
| oaire.citation.title | Food Bioscience | |
| oaire.citation.volume | 71 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Liberti | |
| person.givenName | Davide | |
| person.identifier.orcid | 0000-0003-1255-5030 | |
| relation.isAuthorOfPublication | e0b6df0b-ba93-450a-93f7-ec0936394157 | |
| relation.isAuthorOfPublication.latestForDiscovery | e0b6df0b-ba93-450a-93f7-ec0936394157 |