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Melatonin rhythms beyond the pineal organ: gene expression of receptors and biosynthesis enzymes in wild and F1 Senegalese sole

2025-05-01Journal article Open access
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dc.contributor.authorFélix de Azeredo Pinto e Melo, Francisca
dc.contributor.authorOliveira, Catarina
dc.contributor.authorMartín, Ignacio
dc.contributor.authorManchado, Manuel
dc.contributor.authorSánchez-Vázquez, Francisco J.
dc.contributor.authorVera, Luisa M.
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2025-05-23T10:10:08Z
dc.date.available2025-05-23T10:10:08Z
dc.date.issued2025-05-01
dc.description.abstractFish gonadal melatonin production is still unexplored and could contribute to a better understanding of its role in reproduction control, especially for species with reproductive impairments. This study aimed to comprehend if Senegalese sole testes are an extra-pineal production site of melatonin and if it has seasonal and daily variations. Wild and F1 broodstocks were sampled in the breeding season (BS) and out of the reproductive season (OS), at mid-light (ML) and mid-dark (MD) daytimes. Blood plasma melatonin concentration was determined by radioimmunoassay (RIA). The expression of genes involved in melatonin biosynthesis (tph1a, tph2, hiomt1, aanat1a, aanat1b, and aanat2) and melatonin receptors (mel1, mel1c, and mel2) was evaluated in the brain, eye, and testis by quantitative real-time PCR (qPCR). Plasma melatonin concentration in wild sole displayed day/night differences in both seasons (average ML: 36 +/- 22 pg/mL, MD: 108 +/- 63 pg/mL), whereas differences in the F1 broodstock were only found OS (ML: 100 +/- 54 pg/mL, MD: 187 +/- 88 pg/mL). Gene expression of mel1 and mel2 receptors, and tph1a, aanat1a, aanat2, and hiomt1 enzymes was detected and quantified in the fish testes. Moreover, daily and seasonal fluctuations in the expression of those genes were found in all tissues and broodstock groups. However, the F1 group showed distinct gene expression patterns compared to the wild type, suggesting a disruption in the circadian system. This study revealed that Senegalese sole testes are a melatonin production site and, at the same time, suggested a dysregulation in the hypothalamus-pituitary-gonad (HPG) axis of F1 males.eng
dc.description.sponsorshipCOVID/BD/153473/2023; 57/2016/CP1361/CT0007;
dc.identifier.doi10.1007/s10695-025-01502-1
dc.identifier.eissn1573-5168
dc.identifier.issn0920-1742
dc.identifier.urihttp://hdl.handle.net/10400.1/27158
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofFish Physiology and Biochemistry
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSolea senegalensis
dc.subjectMelatonin receptors
dc.subjectExtra-pineal production
dc.subjectRt-PCR
dc.titleMelatonin rhythms beyond the pineal organ: gene expression of receptors and biosynthesis enzymes in wild and F1 Senegalese soleeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148280%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue3
oaire.citation.startPage88
oaire.citation.titleFish Physiology and Biochemistry
oaire.citation.volume51
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameOliveira
person.familyNameCabrita
person.givenNameFrancisca
person.givenNameCatarina
person.givenNameElsa
person.identifier.ciencia-idAC1C-FB89-E69C
person.identifier.ciencia-id0816-30C5-51AC
person.identifier.orcid0000-0002-6679-761X
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id35433190500
person.identifier.scopus-author-id26645697000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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