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Five phylogenetic groups identified in the coat protein gene of grapevine leafroll-associated virus 3 obtained from Portuguese grapevine varieties

dc.contributor.authorGouveia, P.
dc.contributor.authorSantos, M. T.
dc.contributor.authorEiras Dias, J. E.
dc.contributor.authorNolasco, Gustavo
dc.date.accessioned2015-06-15T14:13:47Z
dc.date.available2015-06-15T14:13:47Z
dc.date.issued2011
dc.description.abstractThe genetic variability and population structure of grapevine leafroll-associated virus 3 (GLRaV-3) variants were updated by examining the diversity within the viral coat protein (CP) gene among 174 isolates belonging to a collection of Vitis vinifera representing most of the Portuguese varieties. Phylogenetic analysis revealed the existence of five well-defined clusters. Three of these correspond to previously defined groups, another corresponds to variants from Chile for which only one sequence has been previously identified, and an additional new group includes only Portuguese variants. A typing tool based on asymmetric PCR-ELISA (APET) was developed within the frame of this population structure. This tool was used to assess the prevalence of each phylogenetic group among the infected grapevine varieties. Although most of the isolates harbour variants from groups 1 and 2, variants from the remaining three groups exist in a number of varieties, reinforcing the notion that they are genuine genomic variants and are not isolated, atypical cases.
dc.identifier.doihttps://dx.doi.org/10.1007/s00705-010-0878-7
dc.identifier.issn0304-8608
dc.identifier.issn1432-8798
dc.identifier.otherAUT: GNO00324;
dc.identifier.urihttp://hdl.handle.net/10400.1/6246
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relation.isbasedonP-002-TRA
dc.titleFive phylogenetic groups identified in the coat protein gene of grapevine leafroll-associated virus 3 obtained from Portuguese grapevine varieties
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage420
oaire.citation.startPage413
oaire.citation.titleArchives of Virology
oaire.citation.volume156
person.familyNameNolasco
person.givenNameGustavo
person.identifier.orcid0000-0002-7025-6023
person.identifier.scopus-author-id6602625528
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication87a8035f-bc15-44dd-b8c3-4288c5660fff
relation.isAuthorOfPublication.latestForDiscovery87a8035f-bc15-44dd-b8c3-4288c5660fff

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