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Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia

2010-11Journal article Open access
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dc.contributor.authorGomes, Newton C. M.
dc.contributor.authorFlocco, Cecilia G.
dc.contributor.authorCosta, Rodrigo
dc.contributor.authorJunca, Howard
dc.contributor.authorVilchez, Ramiro
dc.contributor.authorPieper, Dietmar H.
dc.contributor.authorKroegerrecklenfort, Ellen
dc.contributor.authorParanhos, Rodolfo
dc.contributor.authorMendonca-Hagler, Leda C. S.
dc.contributor.authorSmalla, Kornelia
dc.date.accessioned2018-12-07T14:57:59Z
dc.date.available2018-12-07T14:57:59Z
dc.date.issued2010-11
dc.description.abstractIn this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched.
dc.description.sponsorshipDeutsche Forschungsgemeinschaft [SM59/4-1, 4-2]; FAPERJ-Brazil; European Commission [003998, 211684]; Alexander-von-Humboldt-Stiftung; CONICET (Argentina)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1111/j.1574-6941.2010.00962.x
dc.identifier.issn0168-6496
dc.identifier.urihttp://hdl.handle.net/10400.1/11800
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley-Blackwell Publishing, Inc
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGradient gel-electrophoresis
dc.subjectComplete nucleotide-sequence
dc.subjectHost-range plasmids
dc.subjectBacterial communities
dc.subjectAcinetobacter-calcoaceticus
dc.subjectPseudomonas-Aeruginosa
dc.subjectMicrobial communities
dc.subjectMultivariate analyses
dc.subjectResistance plasmids
dc.subjectDioxygenase genes
dc.titleMangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage290
oaire.citation.issue2
oaire.citation.startPage276
oaire.citation.titleFEMS Microbiology Ecology
oaire.citation.volume74
person.familyNameda Silva Costa
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7203063627
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication4495127c-16f2-4231-9fc1-b471c661036b
relation.isAuthorOfPublication.latestForDiscovery4495127c-16f2-4231-9fc1-b471c661036b

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