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Proteomic analysis of the mucus of the photosynthetic sea slug Elysia crispata

2024Journal article Open access
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dc.contributor.authorLopes, Diana
dc.contributor.authorAveiro, Susana
dc.contributor.authorCruz, Sónia
dc.contributor.authorCartaxana, Paulo
dc.contributor.authorDomingues, Pedro
dc.date.accessioned2024-03-21T10:15:09Z
dc.date.available2024-03-21T10:15:09Z
dc.date.issued2024
dc.description.abstractElysia crispata is a tropical sea slug that can retain intracellular functional chloroplasts from its algae prey, a mechanism termed kleptoplasty. This sea slug, like other gastropods, secretes mucus, a viscous secretion with multiple functions, including lubrication, protection, and locomotion. This study presents the first comprehensive analysis of the mucus proteome of the sea slug E. crispata using gel electrophoresis and HPLC-MS/MS. We identified 306 proteins in the mucus secretions of this animal, despite the limited entries for E. crispata in the Uniprot database. The functional annotation of the mucus proteome using Gene Ontology identified proteins involved in different functions such as hydrolase activity (molecular function), carbohydrate-derived metabolic processes (biological processes) and cytoskeletal organization (cell component). Moreover, a high proportion of proteins with enzymatic activity in the mucus of E. crispata suggests potential biotechnological applications including antimicrobial and antitumor activities. Putative antimicrobial properties are reinforced by the high abundance of hydrolases. This study also identified proteins common in mucus samples from various species, supporting a common mechanism of mucus in protecting cells and tissues while facilitating animal movement. Significance: Marine species are increasingly drawing the interest of researchers for their role in discovering new bioactive compounds. The study "Proteomic Analysis of the Mucus of the Photosynthetic Sea Slug Elysia crispata" is a pioneering effort that uncovers the complex protein content in this fascinating sea slug's mucus. This detailed proteomic study has revealed proteins with potential use in biotechnology, particularly for antimicrobial and antitumor purposes. This research is a first step in exploring the possibilities within the mucus of Elysia crispata, suggesting the potential for new drug discoveries. These findings could be crucial in developing treatments for severe diseases, especially those caused by multidrug-resistant bacteria, and may lead to significant advances in medical research.pt_PT
dc.description.sponsorshipPOCI- 01-0247-FEDER-035234; LISBOA-01-0247-FEDER-035234; ALG-01-0247-FEDER-035234pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jprot.2024.105087pt_PT
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10400.1/20526
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationKleptoplasty: The sea slug that got away with stolen chloroplasts
dc.relationMucus of Sacoglossa sea slugs: Contribution of kleptoplast photosynthesis, biochemical characterization and adhesive properties
dc.relationRole of Kleptoplasty in photosynthetic Sacoglossan sea slugs metabolism (RoKS)
dc.relationNot Available
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectElysia crispatapt_PT
dc.subjectKleptoplastypt_PT
dc.subjectMucuspt_PT
dc.subjectProteomicspt_PT
dc.subjectHPLC-MS/MSpt_PT
dc.subjectBlue biotechnologypt_PT
dc.titleProteomic analysis of the mucus of the photosynthetic sea slug Elysia crispatapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleKleptoplasty: The sea slug that got away with stolen chloroplasts
oaire.awardTitleMucus of Sacoglossa sea slugs: Contribution of kleptoplast photosynthesis, biochemical characterization and adhesive properties
oaire.awardTitleRole of Kleptoplasty in photosynthetic Sacoglossan sea slugs metabolism (RoKS)
oaire.awardTitleNot Available
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/949880/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2020.08672.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.03278.CEECIND%2FCP1589%2FCT0012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F01434%2F2018%2FCP1559%2FCT0003/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0094%2F2020/PT
oaire.citation.startPage105087pt_PT
oaire.citation.titleJournal of Proteomicspt_PT
oaire.citation.volume294pt_PT
oaire.fundingStreamH2020
oaire.fundingStreamPOR_CENTRO
oaire.fundingStreamCEEC IND 3ed
oaire.fundingStreamCEEC IND 2018
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAveiro
person.givenNameSusana
person.identifier.ciencia-idD312-07B4-DA74
person.identifier.orcid0000-0002-6651-3226
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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