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No induction of antimicrobial resistance in Staphylococcus aureus and Listeria monocytogenes during continuous exposure to eugenol and citral

2014-05Journal article Open access
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dc.contributor.authorApolonio, Joana
dc.contributor.authorFaleiro, Maria L.
dc.contributor.authorMiguel, Maria Graca
dc.contributor.authorNeto, Luis
dc.date.accessioned2018-12-07T14:53:49Z
dc.date.available2018-12-07T14:53:49Z
dc.date.issued2014-05
dc.description.abstractThe aim of this study was to evaluate the adaptation response of Staphylococcus aureus, methicillin-resistant S.aureus (MRSA), and Listeria monocytogenes to the essential oil (EO), eugenol, and citral. The minimum inhibitory concentration of eugenol and citral was determined by agar dilution and microdilution. Adaptation to eugenol and citral was done by sequential exposure of the pathogens to increasing concentrations of the essential oils. The M2-A9 standard was used to determine the antibiotic susceptibility. The effect of eugenol and citral on the adherence ability was evaluated by the crystal violet assay. The impact of adaptation to eugenol on virulence was estimated using the Galleria mellonella model. No development of resistance to the components and antibiotics was observed in the adapted cells of S.aureus, MRSA, and L.monocytogenes. Eugenol and citral at subinhibitory concentration reduced the bacterial adherence. Adaptation to subinhibitory concentration of eugenol affected the virulence potential of S.aureus, MRSA, and L.monocytogenes. Eugenol and citral do not pose a risk of resistance development in a continuous mode of use. These EO components showed a high efficacy as antistaphylococcal and antilisterial biofilm agents. Adaptation at subinhibitory concentration of eugenol protected the larvae against listerial and staphylococcal infection.
dc.description.sponsorshipFCT - Fundacao para a Ciencia e Tecnologia [PEst-OE/EQB/LA0023/2013]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1111/1574-6968.12440
dc.identifier.issn0378-1097
dc.identifier.issn1574-6968
dc.identifier.urihttp://hdl.handle.net/10400.1/11705
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford University Press
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTea tree oil
dc.subjectEscherichia-Coli
dc.subjectMelaleuca-Alternifolia
dc.subjectGalleria-Mellonella
dc.subjectAntibiotic-resistance
dc.subjectSublethal concentrations
dc.subjectStress resistance
dc.subjectAlpha-terpineol
dc.subjectAcid tolerance
dc.subjectSalt stress
dc.titleNo induction of antimicrobial resistance in Staphylococcus aureus and Listeria monocytogenes during continuous exposure to eugenol and citral
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage101
oaire.citation.issue2
oaire.citation.startPage92
oaire.citation.titleFEMS Microbiology Letters
oaire.citation.volume354
person.familyNameApolónio
person.familyNameFaleiro
person.familyNameMiguel
person.familyNameNeto
person.givenNameJoana
person.givenNameMaria Leonor
person.givenNameMaria
person.givenNameLuís
person.identifier252666
person.identifier2096804
person.identifier.ciencia-id791E-9E39-2795
person.identifier.ciencia-id281D-5E01-F09A
person.identifier.ciencia-id2D16-F3CF-DDF3
person.identifier.orcid0000-0002-9727-1813
person.identifier.orcid0000-0002-3878-6948
person.identifier.orcid0000-0002-1601-5501
person.identifier.orcid0000-0003-1834-6459
person.identifier.scopus-author-id6507927018
person.identifier.scopus-author-id9941792100
person.identifier.scopus-author-id15838061200
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication5361e29a-3b79-4ce8-9f87-0c667d60588d
relation.isAuthorOfPublicationecda84a3-0649-4504-abb0-9c4c56ff862c
relation.isAuthorOfPublicationf2d75629-4aa7-4d98-bbfa-ef9350937bd4
relation.isAuthorOfPublication.latestForDiscovery5361e29a-3b79-4ce8-9f87-0c667d60588d

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