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Extracting protein from microalgae (Tetraselmis chuii) for proteome analysis

2022Journal article Open access
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dc.contributor.authorL, Anjos
dc.contributor.authorEstêvão, J.
dc.contributor.authorInfante, Carlos
dc.contributor.authorMantecón, Lalia
dc.contributor.authorPower, Deborah Mary
dc.date.accessioned2022-07-25T11:29:10Z
dc.date.available2022-07-25T11:29:10Z
dc.date.issued2022
dc.description.abstractMicroalgae have high potential as a resource for sustainable and green protein for food or bioactive molecules. Nonetheless, despite the high protein content of microalgae (40 - 70% dry weight) progress in the characterization of their protein composition remains challenging. This is due to the highly variable chemical composition of microalgae strains and factors such as their rigid thick cell wall, polysaccharide content, protein stability, pH. The method described herein was developed to optimize protein extraction for proteome analysis of microalgae (Tetraselmis chuii) biomass. The effects on protein solubility of solvent type (organic, denaturing, and non-denaturing) combined with three customized microalgae disruption methods were investigated. The proteome targeted high quality protein extracts were for hydro-soluble proteins recovered by cell disruption using bead milling coupled to centrifugation (protein yield approximate to 13%). The developed method is inexpensive, efficient (yielding high-quality protein extracts with a low content of interfering compounds) and from an industrial perspective easily scalable and compatible with other applications. To add value to the end product we additionally propose the use of stabilizing agents to maintain protein solubility during refrigerated storage and a method targeting the fractionation of low molecular weight proteins. An inexpensive easy-to-do 5 step protocol for microalgae protein extracts. A protein extraction method free from dangerous or highly polluting chemicals. Production of high yield aqueous protein extracts suitable for proteomics. (C) 2022 The Authors. Published by Elsevier B.V.pt_PT
dc.description.sponsorshipEMBRC.PT ALG-01-0145-FEDER-022121
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.mex.2022.101637pt_PT
dc.identifier.eissn2215-0161
dc.identifier.urihttp://hdl.handle.net/10400.1/18084
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNot Available
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectProtein extractionpt_PT
dc.subjectMicroalgaept_PT
dc.subjectProteomept_PT
dc.subjectProteomicspt_PT
dc.titleExtracting protein from microalgae (Tetraselmis chuii) for proteome analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F04462%2F2017%2FCP1387%2FCT0027/PT
oaire.citation.startPage101637pt_PT
oaire.citation.titleMethodsXpt_PT
oaire.citation.volume9pt_PT
oaire.fundingStreamDL 57/2016
oaire.fundingStreamCEEC IND 2017
person.familyNameAnjos Guerreiro
person.familyNameEstêvão
person.familyNameInfante
person.familyNamePower
person.givenNameLiliana Isabel Tomé
person.givenNameJoão
person.givenNameCarlos
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0001-6201-7180
person.identifier.orcid0000-0002-6158-9344
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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