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Genomic DNA isolation from green and brown algae (Caulerpales and Fucales) for microsatellite library construction

2006-06Journal article Restricted access
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dc.contributor.authorE, Varela-Álvarez
dc.contributor.authorAndreakis, N.
dc.contributor.authorLago-Lestón, Asunción
dc.contributor.authorPearson, G. A.
dc.contributor.authorSerrão, Ester
dc.contributor.authorProcaccini, G.
dc.contributor.authorDuarte, C. M.
dc.contributor.authorMarbà, N.
dc.date.accessioned2014-06-03T13:02:55Z
dc.date.available2014-06-03T13:02:55Z
dc.date.issued2006-06
dc.date.updated2014-05-21T13:40:29Z
dc.description.abstractA method for isolating high-quality DNA is presented for the green algae Caulerpa sp. (C. racemosa, C. prolifera, and C. taxifolia) and the brown alga Sargassum muticum. These are introduced, and invasive species in Europe, except for the native C. prolifera. Previous methods of extraction, using cetyl trimethyl ammonium bromide or various commercial kits, were used to isolate genomic DNA but either no DNA or DNA of very low quality was obtained. Genomic libraries were attempted with Caulerpa sp. on three occasions and either the restriction enzyme, the Taq polymerase, or the T4 ligase was inhibited, probably by the large amount of polysaccharides in these algae. The method presented here consists of the rapid isolation of stable nuclei, followed by DNA extraction. Yields of 6–10 μg genomic DNA from 1 g fresh blades were obtained. After genomic DNA was isolated from fresh material, the quality was checked by agarose gel. Quantification of DNA concentration was performed using UV spectrophotometric measurement of the A260/A280 ratio. The DNA was suitable for PCR, cloning, and hybridization. The DNA isolated using this method allowed successful construction of microsatellite libraries for Caulerpa species and S. muticum. The technique is inexpensive and appropriate for the isolation of multiple samples of DNA from a small amount of fresh material.por
dc.identifier.citationVarela-Álvarez, E.; Andreakis, N.; Lago-Lestón, A.; Pearson, G.A.; Serrão, E.A.; Procaccini, G.; Duarte, C.M.; Marbá, N.Genomic DNA isolation from green and brown algae (Caulerpales and Fucales) for microsatellite library construction, Journal of Phycology, 42, 3, 741-745, 2006.por
dc.identifier.doihttp://dx.doi.org/ 10.1111/j.1529-8817.2006.00218.x
dc.identifier.issn0022-3646
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/4159
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPhycological Society of Americapor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/j.1529-8817.2006.00218.x/abstractpor
dc.subjectAlgaepor
dc.subjectCaulerpapor
dc.subjectCTABpor
dc.subjectDNA extractionpor
dc.subjectMicrosatellitepor
dc.subjectNuclei isolationpor
dc.subjectPopulation geneticspor
dc.subjectSargassumpor
dc.subjectSeaweedpor
dc.titleGenomic DNA isolation from green and brown algae (Caulerpales and Fucales) for microsatellite library constructionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage745por
oaire.citation.issue3por
oaire.citation.startPage741por
oaire.citation.titleJournal of Phycologypor
oaire.citation.volume42por
person.familyNameVarela-Álvarez
person.familyNameLago-Lestón
person.familyNamePearson
person.familyNameSerrao
person.givenNameElena
person.givenNameAsunción
person.givenNameGareth Anthony
person.givenNameEster A.
person.identifier113536
person.identifierC-6686-2012
person.identifier.ciencia-id3315-9919-1A52
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.orcid0000-0001-7262-3475
person.identifier.orcid0000-0003-4361-7732
person.identifier.orcid0000-0002-0768-464X
person.identifier.orcid0000-0003-1316-658X
person.identifier.ridM-4495-2013
person.identifier.ridJ-3911-2013
person.identifier.scopus-author-id6507326527
person.identifier.scopus-author-id57193972525
person.identifier.scopus-author-id55916875600
person.identifier.scopus-author-id7004093604
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublicationcf3375ea-836f-4de2-9cd6-fde518b26838
relation.isAuthorOfPublication0a10c448-c7f5-4c3b-9488-3917c707e35e
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublication.latestForDiscovery45ccfe90-155c-4d6f-9e86-8f0fd064005f

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