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Polyhydroxyfullerene binds cadmium ions and alleviates metal-induced oxidative stress in saccharomyces cerevisiae

2014-09Journal article Open access
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dc.contributor.authorPradhan, Arunava
dc.contributor.authorPinheiro, José Paulo
dc.contributor.authorSeena, Sahadevan
dc.contributor.authorPascoal, Claudia
dc.contributor.authorCassio, Fernanda
dc.date.accessioned2018-12-07T14:58:12Z
dc.date.available2018-12-07T14:58:12Z
dc.date.issued2014-09
dc.description.abstractThe water-soluble polyhydroxyfullerene (PHF) is a functionalized carbon nanomaterial with several industrial and commercial applications. There have been controversial reports on the toxicity and/or antioxidant properties of fullerenes and their derivatives. Conversely, metals have been recognized as toxic mainly due to their ability to induce oxidative stress in living organisms. We investigated the interactive effects of PHF and cadmium ions (Cd) on the model yeast Saccharomyces cerevisiae by exposing cells to Cd (<= 5 mg liter(-1)) in the absence or presence of PHF (<= 500 mg liter(-1)) at different pHs (5.8 to 6.8). In the absence of Cd, PHF stimulated yeast growth up to 10.4%. Cd inhibited growth up to 79.7%, induced intracellular accumulation of reactive oxygen species (ROS), and promoted plasma membrane disruption in a dose- and pH-dependent manner. The negative effects of Cd on growth were attenuated by the presence of PHF, and maximum growth recovery (53.8%) was obtained at the highest PHF concentration and pH. The coexposure to Cd and PHF decreased ROS accumulation up to 36.7% and membrane disruption up to 30.7% in a dose- and pH-dependent manner. Two mechanisms helped to explain the role of PHF in alleviating Cd toxicity to yeasts: PHF decreased Cd-induced oxidative stress and bound significant amounts of Cd in the extracellular medium, reducing its bioavailability to the cells.
dc.description.sponsorshipFEDER-POFC-COMPETE; Portuguese Foundation for Science and Technology [PEst-OE/BIA/UI4050/2014, PTDC/AAC-AMB/121650/2010]; [SFRH/BD/45614/2008]; [Pest-OE/EQB/LA0023/2013]
dc.identifier.doi10.1128/AEM.01329-14
dc.identifier.issn0099-2240
dc.identifier.issn1098-5336
dc.identifier.urihttp://hdl.handle.net/10400.1/11911
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmer Soc Microbiology
dc.subjectCell death
dc.subjectMitochondrial dysfunction
dc.subjectFullerenol C-60(Oh)(24)
dc.subjectScavenging activity
dc.subjectLipid peroxidation
dc.subjectCortical neurons
dc.subjectYeast
dc.subjectNanoparticles
dc.subjectAccumulation
dc.subjectInvolvement
dc.titlePolyhydroxyfullerene binds cadmium ions and alleviates metal-induced oxidative stress in saccharomyces cerevisiae
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FBIA%2FUI4050%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FAAC-AMB%2F121650%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F45614%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.citation.endPage5881
oaire.citation.issue18
oaire.citation.startPage5874
oaire.citation.titleApplied and Environmental Microbiology
oaire.citation.volume80
oaire.fundingStream5876
oaire.fundingStreamCOMPETE
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
person.familyNamePinheiro
person.givenNameJosé Paulo
person.identifier.orcid0000-0001-7925-9733
person.identifier.ridE-8402-2013
person.identifier.scopus-author-id35838299700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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