dc.contributor.author	Leite, Ricardo
dc.contributor.author	Milan, Massimo
dc.contributor.author	Coppe, Alessandro
dc.contributor.author	Bortoluzzi, Stefania
dc.contributor.author	Dos Anjos, António
dc.contributor.author	Reinhardt, Richard
dc.contributor.author	Saavedra, Carlos
dc.contributor.author	Patarnello, T.
dc.contributor.author	Cancela, Leonor
dc.contributor.author	Bargelloni, Luca
dc.date.accessioned	2014-05-07T14:50:41Z
dc.date.available	2014-05-07T14:50:41Z
dc.date.issued	2013-10-29
dc.date.updated	2014-04-05T17:10:27Z
dc.description.abstract	Background: The Grooved Carpet shell clam Ruditapes decussatus is the autochthonous European clam and the most appreciated from a gastronomic and economic point of view. The production is in decline due to several factors such as Perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam Ruditapes philippinarum. After we sequenced R. decussatus transcriptome we have designed an oligo microarray capable of contributing to provide some clues on molecular response of the clam to Perkinsiosis. Results: A database consisting of 41,119 unique transcripts was constructed, of which 12,479 (30.3%) were annotated by similarity. An oligo-DNA microarray platform was then designed and applied to profile gene expression in R. decussatus heavily infected by Perkinsus olseni. Functional annotation of differentially expressed genes between those two conditionswas performed by gene set enrichment analysis. As expected, microarrays unveil genes related with stress/infectious agents such as hydrolases, proteases and others. The extensive role of innate immune system was also analyzed and effect of parasitosis upon expression of important molecules such as lectins reviewed.Conclusions: This study represents a first attempt to characterize Ruditapes decussatus transcriptome, an important marine resource for the European aquaculture. The trancriptome sequencing and consequent annotation will increase the available tools and resources for this specie, introducing the possibility of high throughput experiments such as microarrays analysis. In this specific case microarray approach was used to unveil some important aspects of host-parasite interaction between the Carpet shell clam and Perkinsus, two non-model species, highlighting some genes associated with this interaction. Ample information was obtained to identify biological processes significantly enriched among differentially expressed genes in Perkinsus infected versus non-infected gills. An overview on the genes related with the immune system on R. decussatus transcriptome is also reported.	por
dc.description.version	info:eu-repo/semantics/publishedVersion
dc.identifier.citation	BMC Genomics. 2013 Oct 29;14(1):741	por
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2164-14-741
dc.identifier.other	AUT: LCA00739;
dc.identifier.uri	http://hdl.handle.net/10400.1/3798
dc.language.iso	eng	por
dc.peerreviewed	yes	por
dc.publisher	BioMed Central	por
dc.rights.holder	Ricardo B Leite et al.; licensee BioMed Central Ltd.
dc.title	mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditapes decussatus: a functional approach to unravel host -parasite interaction	por
dc.type	journal article
dspace.entity.type	Publication
oaire.awardURI	info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F91518%2F2012/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F30112%2F2006/PT
oaire.citation.endPage	17	por
oaire.citation.issue	1:741	por
oaire.citation.startPage	1	por
oaire.citation.title	BMC Genomics	por
oaire.citation.volume	14	por
oaire.fundingStream	SFRH
oaire.fundingStream	SFRH
person.familyName	Leite
person.familyName	Anjos
person.familyName	Cancela
person.givenName	Ricardo
person.givenName	António
person.givenName	M. Leonor
person.identifier	447622
person.identifier.ciencia-id	F115-256E-C84D
person.identifier.ciencia-id	2D11-C8C8-D8EA
person.identifier.orcid	0000-0002-9622-3895
person.identifier.orcid	0000-0001-6521-8930
person.identifier.orcid	0000-0003-3114-6662
person.identifier.rid	B-3389-2008
person.identifier.rid	M-7604-2013
person.identifier.scopus-author-id	8362650900
person.identifier.scopus-author-id	25638237700
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	por
rcaap.type	article	por
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mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditapes decussatus: a functional approach to unravel host -parasite interaction

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