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Contamination analysis of Arctic ice samples as planetary field analogs and implications for future life-detection missions to Europa and Enceladus

dc.contributor.authorCoelho, Lígia F.
dc.contributor.authorBlais, Marie-Amélie
dc.contributor.authorMatveev, Alex
dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorVincent, Warwick F.
dc.contributor.authorDa Silva Costa, Rodrigo
dc.contributor.authorMartins, Zita
dc.contributor.authorCanário, João
dc.date.accessioned2022-12-19T11:44:52Z
dc.date.available2022-12-19T11:44:52Z
dc.date.issued2022
dc.description.abstractMissions to detect extraterrestrial life are being designed to visit Europa and Enceladus in the next decades. The contact between the mission payload and the habitable subsurface of these satellites involves significant risk of forward contamination. The standardization of protocols to decontaminate ice cores from planetary field analogs of icy moons, and monitor the contamination in downstream analysis, has a direct application for developing clean approaches crucial to life detection missions in these satellites. Here we developed a comprehensive protocol that can be used to monitor and minimize the contamination of Arctic ice cores in processing and downstream analysis. We physically removed the exterior layers of ice cores to minimize bioburden from sampling. To monitor contamination, we constructed artificial controls and applied culture-dependent and culture-independent techniques such as 16S rRNA amplicon sequencing. We identified 13 bacterial contaminants, including a radioresistant species. This protocol decreases the contamination risk, provides quantitative and qualitative information about contamination agents, and allows validation of the results obtained. This study highlights the importance of decreasing and evaluating prokaryotic contamination in the processing of polar ice cores, including in their use as analogs of Europa and Enceladus.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-022-16370-5pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/18658
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Portfoliopt_PT
dc.relationAstrobiology analyses of icy biomes as a proxy for extra-terrestrial life in icy moons
dc.relationCentro de Química Estrutural
dc.relationCentro de Química Estrutural
dc.relationOrigin, composition, evolution and exploration of Phobos
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute of Molecular Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleContamination analysis of Arctic ice samples as planetary field analogs and implications for future life-detection missions to Europa and Enceladuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAstrobiology analyses of icy biomes as a proxy for extra-terrestrial life in icy moons
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleOrigin, composition, evolution and exploration of Phobos
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute of Molecular Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F139840%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00100%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/MIT-EXPL%2FSOE%2F0058%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FFIS-AST%2F29932%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0056%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameda Silva Costa
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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