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Spectroscopic Methods for Quantifying Gabapentin: Framing the Methods without Derivatization and Application to Different Pharmaceutical Formulations

2017-11Journal article Restricted
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dc.contributor.authorFonseca, Frederico
dc.contributor.authorde Barros, Ricardo Brito
dc.contributor.authorIlharco, Laura M.
dc.contributor.authorGarcia, Ana
dc.date.accessioned2019-11-20T15:07:14Z
dc.date.available2019-11-20T15:07:14Z
dc.date.issued2017-11
dc.description.abstractThis work aimed at analyzing the performance of direct spectroscopic methods for the quantification of gabapentin (GABAp), given the lack of previous studies, in comparison with the more reviewed and complex derivatization techniques, discussing their susceptibility to the pharmaceutical formulations. All of the methods analyzed showed high selectivity for this pharmaceutical analyte, with recoveries close to 100%. Absorption spectroscopy without derivatization yielded better sensitivity and lower limits of detection and quantification of gabapentin in aqueous solution (AqSol method) when compared with other solvents, such as acidic solution or ethanol/water mixture. Derivatization with sodium hypochlorite presented the highest precision, whereas derivatization with vanillin exhibited the highest accuracy. The best method for GABAp quantification in terms of highest sensitivity, lowest limits of detection, and quantification, and also with good precision and accuracy, proved to be fluorescence with derivatization by 4-chloro-7-nitrobenzofurazan. The effect of the pharmaceutical formulation (nature of excipients) was tested for the most robust and sensitive methods, with and without derivatization, on capsules of five commercial brands. Recoveries in the range of 97.9-101.5% proved that there are no matrix interfering effects. Although not presenting the best performance in all the parameters evaluated, the AqSol method, due to its simplicity, proved to be suitable for the quantification of GABAp in capsules and tables containing the molecule as the active ingredient.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1177/0003702817716181
dc.identifier.issn0003-7028
dc.identifier.issn1943-3530
dc.identifier.urihttp://hdl.handle.net/10400.1/12939
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSAGE Publications
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnalytical method validation
dc.subjectHuman plasma
dc.subjectSpectrofluorometric determination
dc.subjectFluorescence detection
dc.subjectLiquid-chromatography
dc.subjectRapid quantification
dc.subjectAntiepileptic drugs
dc.subjectMass-spectrometry
dc.subjectPractical guide
dc.subjectDosage forms
dc.titleSpectroscopic Methods for Quantifying Gabapentin: Framing the Methods without Derivatization and Application to Different Pharmaceutical Formulations
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FNAN%2F50024%2F2013/PT
oaire.citation.endPage2531
oaire.citation.issue11
oaire.citation.startPage2519
oaire.citation.titleApplied Spectroscopy
oaire.citation.volume71
oaire.fundingStream5876
person.familyNameGarcia
person.givenNameAna
person.identifier.ciencia-idB214-EA2D-350A
person.identifier.orcid0000-0001-6289-4554
person.identifier.scopus-author-id7404608764
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicatione49828e5-432e-46e3-a7fc-2fdbf51a237b
relation.isAuthorOfPublication.latestForDiscoverye49828e5-432e-46e3-a7fc-2fdbf51a237b
relation.isProjectOfPublication42a1b47f-c066-4905-9630-19ce8718d1be
relation.isProjectOfPublication.latestForDiscovery42a1b47f-c066-4905-9630-19ce8718d1be

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