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Localization and distribution of nitric oxide synthase and other neuronal markers in the podia ofHolothuria arguinensis

2020-07Journal article Open access
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dc.contributor.authorMarquet, Nathalie
dc.contributor.authorCanario, Adelino
dc.contributor.authorPower, Deborah
dc.date.accessioned2021-06-24T11:35:17Z
dc.date.available2021-06-24T11:35:17Z
dc.date.issued2020-07
dc.description.abstractThe organization of the nervous system of the holothurian podia-the tentacles, papillae, and tube feet-is still poorly understood, which limits the development of functional studies. Knowledge of nitric oxide (NO) signaling in sea cucumbers is nonexistent, although it is known to play an important role in many essential biological functions, including neurotransmission, throughout the animal kingdom. The objective of this study was to characterize the holothurian podia inHolothuria arguinensis. To this end, we used classical histology, nitric oxide synthase (NOS) distribution, using NADPH-diaphorase histochemistry and NOS immunostaining, and neuronal immunohistochemistry. Our results revealed an abundant distribution of NO in the nervous components of the holothurian podia, suggesting an important role for NO as a neuronal messenger in these structures. Nitrergic fibers were intensely labeled in the longitudinal nerve and the nerve plexus surrounding the stem, but were more weakly labeled in the mesothelium. NOS was also found in scattered cell bodies and abundant fibers in the podia terminal end (i.e., the discs in tentacles and tube feet, and the pointed conical structures in the papillae), with evident neuronal projections to the bud surface, especially in the tentacles. The podia terminal end was the most specialized area and was characterized by a specific nervous arrangement, consisting of a distinct nerve plate, rich in cells and fibers containing potential sensory cells staining positively for neuronal markers, which makes this the most likely candidate to be a chemosensory region and an important candidate for future exploration.
dc.description.sponsorshipFundacao para a Ciencia e a TecnologiaPortuguese Foundation for Science and TechnologyEuropean Commission [SFRH/BD/90761/2012, UIDB/04326/2020]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1111/ivb.12298
dc.identifier.issn1077-8306
dc.identifier.urihttp://hdl.handle.net/10400.1/16394
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationSTUDY OF REPRODUCTIVE BIOLOGY AND CHEMICAL COMMUNICATION OF SEA CUCUMBERS HOLOTHURIA ARGUINENSIS AND H. MAMMATA
dc.relationAlgarve Centre for Marine Sciences
dc.subjectHistology
dc.subjectImmunohistochemistry
dc.subjectNADPH-diaphorase
dc.subjectNitric oxide
dc.subjectSea cucumber
dc.subject.otherMarine & Freshwater Biology
dc.titleLocalization and distribution of nitric oxide synthase and other neuronal markers in the podia ofHolothuria arguinensis
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSTUDY OF REPRODUCTIVE BIOLOGY AND CHEMICAL COMMUNICATION OF SEA CUCUMBERS HOLOTHURIA ARGUINENSIS AND H. MAMMATA
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F90761%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.citation.issue3
oaire.citation.startPagee12298
oaire.citation.titleInvertebrate Biology
oaire.citation.volume139
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMarquet
person.familyNameCanario
person.familyNamePower
person.givenNameNathalie
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifierN-6804-2013
person.identifier143624
person.identifier.ciencia-id5613-BBCD-0EFE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-3718-4478
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id57193647721
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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