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Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties

2024Journal article Open access
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dc.contributor.authorCustódio, Luísa
dc.contributor.authorCziáky, Zoltán
dc.contributor.authorCastañeda-Loaiza, Viana
dc.contributor.authorRodrigues, Maria Joao
dc.date.accessioned2024-02-03T12:47:58Z
dc.date.available2024-02-03T12:47:58Z
dc.date.issued2024
dc.description.abstractThis study established a liquid adventitious root culture for Inula crithmoides L. focusing on producing extracts rich in hepatoprotective caffeoylquinic acids through elicitation. Adventitious roots were induced from in vitro-grown plant leaves cultured on MS medium supplemented with 1 mg/L IBA. Growth and secondary metabolites accumulation (phenolics, flavonoids, hydroxycinnamic acids) were monitored weekly to determine the optimal harvesting time. Then, the adventitious roots were elicited for 4 weeks with varying concentrations of yeast extract-YE (50, 100 and 200 mg/L) and extracted with ethanol. The extracts were evaluated for their hepatoprotective effects against ethanol-induced hepatotoxicity and analyzed through high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Roots of greenhouse-cultivated plants were used to compare the results obtained from in vitro -grown adventitious roots. IBA supplementation induced 100% adventitious root formation, with maximum biomass and phenolic accumulation after 4 weeks. Hydroxycinnamic acid levels increased significantly with YE treatment at all the concentrations, particularly in case of chlorogenic acid and di-O-caffeoylquinic acid isomers. The root extracts displayed significant in vitro hepatoprotective effects, particularly at 50 mg/L YE elicitation. Overall, the elicitation of I. crithmoides liquid root cultures with yeast extract offers a promising, efficient, and cost-effective approach for optimizing the production of valuable caffeoylquinic acids having potential pharmaceutical use.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s11240-024-02690-zpt_PT
dc.identifier.issn0167-6857
dc.identifier.urihttp://hdl.handle.net/10400.1/20363
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationAlgarve Centre for Marine Sciences
dc.relationNot Available
dc.relationXtremeCrops: Optimization of the agronomic conditions for the sustainable cultivation of edible halophytes in an integrated multi-trophic aquaculture IMTA system
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGolden samphirept_PT
dc.subjectAsteraceaept_PT
dc.subjectPlant tissue culturept_PT
dc.subjectYeast extractpt_PT
dc.titleEstablishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective propertiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleNot Available
oaire.awardTitleXtremeCrops: Optimization of the agronomic conditions for the sustainable cultivation of edible halophytes in an integrated multi-trophic aquaculture IMTA system
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00425%2F2017%2FCP1391%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/2020.04541.BD/PT
oaire.citation.issue2pt_PT
oaire.citation.startPage59pt_PT
oaire.citation.titlePlant Cell, Tissue and Organ Culture (PCTOC)pt_PT
oaire.citation.volume156pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
oaire.fundingStreamOE
person.familyNameCustódio
person.familyNameCastañeda-Loaiza
person.familyNameRodrigues
person.givenNameLuísa
person.givenNameViana
person.givenNameMaria João
person.identifierR-004-VNG
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.ciencia-id2319-B2D8-AF48
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.orcid0000-0003-4338-7703
person.identifier.orcid0000-0002-8839-1389
person.identifier.orcid0000-0001-8732-710X
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
person.identifier.scopus-author-id56031608100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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