Repository logo
 
Publication

Estudo da distribuiĆ§Ć£o do transportador da hormona tiroxina - transretina - em dourada, Sparus aurata (Linnaeus, 1758)

1998Master thesis Open access
Simple item page
dc.contributor.advisorPower, Deborah
dc.contributor.authorCharrĆ£o, Adelaide da ConceiĆ§Ć£o Dimas Henrique
dc.date.accessioned2015-09-09T12:25:43Z
dc.date.available2015-09-09T12:25:43Z
dc.date.issued1998
dc.date.submitted1998
dc.descriptionDissertaĆ§Ć£o de Mestrado, Aquacultura, Unidade de CiĆŖncias e Tecnologias dos Recursos AquĆ”ticos, Universidade do Algarve, 1998por
dc.description.abstractTransthyretin (TTR) is one of three thyroxine binding proteins found in the serum of higher animais. It has been shown to be synthesised and secreted in the choroid plexus of placental mammals, birds and reptiles but not in amphibians. Recently has cloned transthyretin from a sea bream liver cDNA library (Santos & Power, 1998). The objective of the present study was to determine the distribution of TTR in sea bream and study its regulation thyroid hormone. Ti and T4 (0,1 mg/g body weight) or saline was administered to sea bream by a single intraperitoneal injection. At 6h, I2h and 24h after injection treated and controls fish were killed and tissue removed. Expression of TTR was determined by RT-PCR, using homologous primers coupled with Southern blotting. The identity of PCR products was also confirmed by partial sequencing. TTR was expressed in liver, brain, heart, gill and gut and undetectable in kidney and muscle. Treatment with T3 e T4 changed the levei of expression of TTR in tissue. During the timescale studied there was a different up-regulation ofTTR expression by T4and T3 in liver and brain.por
dc.description.abstractA transretina (TTR) Ć© uma das trĆŖs proteĆ­nas que transporta a tiroxina no plasma sanguĆ­neo de animais superiores. VĆ”rios estudos demonstraram a sua sĆ­ntese e secreĆ§Ć£o no choroid plexus de mamĆ­feros placentĆ rios, aves, rĆ©pteis. Nos anfĆ­bios tal nĆ£o se verifica. Recentemente foi clonado o gene para a transretina a partir de um banco de cDNA de fĆ­gado de dourada (Santos & Power, 1998). O objectivo deste estudo foi determinar a distribuiĆ§Ć£o da transretina em alguns tecidos da dourada e estudar a influĆŖncia das hormonas produzidas pela tirĆ³ide na regulaĆ§Ć£o da transretina. Juvenis de dourada foram injectados intraperitonalmente com e T4 (0,1 mg/gr. peso corporal) ou com soluĆ§Ć£o salina. Seis horas, doze horas e vinte e quatro horas apĆ³s a injecĆ§Ć£o, peixes tratados (com Ti e T4) e controlos foram mortos e os tecidos removidos. A expressĆ£o da transtretina foi determinada por RT-PCR, usando primers homĆ³logos, e por Southern blot. A identidade dos produtos de PCR foi confirmada por sequenciaĆ§Ć£o. VerifĆ­cou-se, neste estudo que a transretina Ć© expressa no fĆ­gado, cĆ©rebro, coraĆ§Ć£o, brĆ¢nquias e intestino e indectĆ”vel no rim e mĆŗsculo. O tratamento com T3 e T4 muda os nĆ­veis de expressĆ£o da transretina nos tecidos. Durante os intervalos de tempo estudados verifĆ­cou-se que a expressĆ£o da transretina Ć© regulada de forma diferente no fĆ­gado e no cĆ©rebro, por acĆ§Ć£o da T3 e T4.por
dc.description.sponsorshipJNICT, Programa Praxis XXIpor
dc.identifier.urihttp://hdl.handle.net/10400.1/6754
dc.language.isoporpor
dc.subjectTransretinapor
dc.subjectTiroxinapor
dc.subjectPeixespor
dc.subjectDouradapor
dc.subjectSparus auratapor
dc.subjectAquaculturapor
dc.titleEstudo da distribuiĆ§Ć£o do transportador da hormona tiroxina - transretina - em dourada, Sparus aurata (Linnaeus, 1758)por
dc.typemaster thesis
dspace.entity.typePublication
rcaap.rightsopenAccesspor
rcaap.typemasterThesispor
thesis.degree.disciplineAquaculturapor
thesis.degree.grantorUniversidade do Algarve, Unidade de CiĆŖncias e Tecnologias dos Recursos AquĆ”ticospor
thesis.degree.levelMestrepor
thesis.degree.nameMestrado em Aquaculturapor

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
S14_CHARRAO--Estudo_da_distribuicao_do_transportador.pdf
Size:
1.43 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UA01-Teses