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ICI 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testis

2006-12-27Journal article Open access
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dc.contributor.authorPinto, Patricia IS
dc.contributor.authorSingh, Pratap B.
dc.contributor.authorCondeça, João B.
dc.contributor.authorTeodósio, H. R.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-16T11:21:53Z
dc.date.available2014-05-16T11:21:53Z
dc.date.issued2006-12-27
dc.date.updated2014-05-13T09:30:04Z
dc.description.abstractICI 182,780 (ICI) belongs to a new class of antiestrogens developed to be pure estrogen antagonists and, in additi on to its therapeutic use, it has been used to knock-out estrogen and estrogen receptor (ER) actions in several mammalian species. In the present study, the effects and mechanism of action of ICI were investigated in the teleost fish, sea bream (Sparus auratus). Three independent in vivo experiments were performed in which mature male tilapia (Oreochromis mossambicus) or sea bream received intra-peritoneal implants containing estradiol-17 beta (E2), ICI or a combination of both compounds. The effects of E2 and ICI on plasma calcium levels were measured and hepatic and testicular gene expression of the three ER subtypes, ER alpha, ER beta a and ER beta b, and the estrogen-responsive genes, vitellogenin II and choriogenin L, were analyzed by semi-quantitative RT-PCR in sea bream. E2 treatment caused an increase in calcium levels in tilapia, while ICI alone had no noticeable effect, as expected. However, pretreatment with ICI synergistically potentiated the effect of E2 on plasma calcium in both species. ICI mimicked some E2 actions in gene expression in sea bream liver upregulating ER alpha, vitellogenin II and choriogenin L, although, unlike E2, it did not downregulate ER beta a and ER beta b. In contrast, no effects of E2 or ICI alone were detected in the expression of ERs in testis, while vitellogenin II and choriogenin L were upregulated by E2 but not ICI. Finally, pretreatment with ICI had a synergistic effect on the hepatic E2 down- regulation of ER beta b, but apparently blocked the ER alpha up-regulation by E2. These results demonstrate that ICI has agonistic effects on several typical estrogenic responses in fish, but its actions are tissue-specific. The mechanisms for the ICI agonistic activity are still unknown; although the ICI induced up-regulation of ER alpha mRNA could be one of the factors contributing to the cellular response.por
dc.identifier.citationPinto, Patricia I. S.; Singh, Pratap B.; Condeca, Joao B.; Teodosio, Helena R.; Power, Deborah M.; Canario, Adelino V. M.ICI 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testis, Reproductive Biology and Endocrinology, 4, 67, sn-sn, 2006.por
dc.identifier.issn1477-7827
dc.identifier.otherhttp://dx.doi.org/10.1186/1477-7827-4-67
dc.identifier.otherAUT: DPO00386; ACA00258
dc.identifier.urihttp://hdl.handle.net/10400.1/3981
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBioMed Centralpor
dc.relationThe estrogen receptor in seabream Sparus aurata reproduction: diversity, expression, mechanisms and physiological actions
dc.relation.publisherversionhttp://www.rbej.com/content/pdf/1477-7827-4-67.pdfpor
dc.titleICI 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testispor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe estrogen receptor in seabream Sparus aurata reproduction: diversity, expression, mechanisms and physiological actions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FCVT%2F39355%2F2001/PT
oaire.citation.endPage11por
oaire.citation.issue67por
oaire.citation.startPage1por
oaire.citation.titleReproductive Biology and Endocrinologypor
oaire.citation.volume4por
oaire.fundingStreamPOCI
person.familyNamePinto
person.familyNamePower
person.familyNameCanario
person.givenNamePatricia IS
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier643457
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-3817-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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