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Isolation and characterisation of metallothionein from the clam Ruditapes decussatus

2003-05Journal article Open access
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dc.contributor.authorSimes, D
dc.contributor.authorBebianno, Maria João
dc.contributor.authorMoura, José J. G.
dc.date.accessioned2018-12-07T14:57:53Z
dc.date.available2018-12-07T14:57:53Z
dc.date.issued2003-05
dc.description.abstractMetallothioneins (MT) were obtained after purification from metal-exposed clams (Ruditapes decussatus) using gel-permeation and ion-exchange chromatography. Four cadmium-metallothioneins (CdMTs) were resolved by ion-exchange chromatography and they all had similar molecular weights, high cadmium content and an absorption spectra indicative of the presence of characteristic Cd-S aggregates. The NH2-terminal sequence suggests the presence of at least two class I clam MT isoforms. For the other two putative clam CdMTs isolated, the results of the amino acid determination were inconclusive. One was slightly contaminated and the other one had a blocked NH2-terminal. These clam metalothioneins contain glycine, which seems to be a common feature of molluscan MT family and exhibited more similarity to oysters than to mussels. Further investigation on the inducibility of these isoforms will be necessary if clams are to be used as biomarkers of metal exposure.
dc.identifier.doi10.1016/S0166-445X(02)00185-6
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/11748
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectMolluscan metallothioneins
dc.subjectMass spectrometry
dc.subjectCrystal structure
dc.subjectPurification
dc.subjectCadmium
dc.subjectAcid
dc.subjectSequences
dc.subjectProteins
dc.subjectBinding
dc.subjectCdna
dc.subjectMetallothionein
dc.subjectClam
dc.subjectRuditapes decussatus
dc.subjectCd
dc.subjectToxicity
dc.titleIsolation and characterisation of metallothionein from the clam Ruditapes decussatus
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage318
oaire.citation.issue3
oaire.citation.startPage307
oaire.citation.titleAquatic Toxicology
oaire.citation.volume63
person.familyNameSimes
person.familyNameBebianno
person.givenNameDina
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0002-5145-4753
person.identifier.orcid0000-0003-1492-8566
person.identifier.ridN-2789-2014
person.identifier.scopus-author-id7201884723
person.identifier.scopus-author-id7004152715
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication31bac96d-1d01-4b8f-8f2b-9da4be31ea41
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery31bac96d-1d01-4b8f-8f2b-9da4be31ea41

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