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Environmental DNA reveals temporal variation in mesophotic reefs of the Humboldt upwelling ecosystems of central Chile: toward a baseline for biodiversity monitoring of unexplored marine habitats

2024Journal article Open access
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dc.contributor.authorSaenz‐Agudelo, Pablo
dc.contributor.authorRamirez, Paula
dc.contributor.authorBeldade, Ricardo
dc.contributor.authorCampoy, Ana N.
dc.contributor.authorGarmendia, Vladimir
dc.contributor.authorSearch, Francesca V.
dc.contributor.authorFernández, Miriam
dc.contributor.authorWieters, Evie A.
dc.contributor.authorNavarrete, Sergio A.
dc.contributor.authorSerrao, Ester
dc.contributor.authorPérez‐Matus, Alejandro
dc.date.accessioned2024-03-22T10:32:09Z
dc.date.available2024-03-22T10:32:09Z
dc.date.issued2024
dc.description.abstractTemperate mesophotic reef ecosystems (TMREs) are among the least known marine habitats. Information on their diversity and ecology is geographically and temporally scarce, especially in highly productive large upwelling ecosystems. Lack of information remains an obstacle to understanding the importance of TMREs as habitats, biodiversity reservoirs and their connections with better-studied shallow reefs. Here, we use environmental DNA (eDNA) from water samples to characterize the community composition of TMREs on the central Chilean coast, generating the first baseline for monitoring the biodiversity of these habitats. We analyzed samples from two depths (30 and 60 m) over four seasons (spring, summer, autumn, and winter) and at two locations approximately 16 km apart. We used a panel of three metabarcodes, two that target all eukaryotes (18S rRNA and mitochondrial COI) and one specifically targeting fishes (16S rRNA). All panels combined encompassed eDNA assigned to 42 phyla, 90 classes, 237 orders, and 402 families. The highest family richness was found for the phyla Arthropoda, Bacillariophyta, and Chordata. Overall, family richness was similar between depths but decreased during summer, a pattern consistent at both locations. Our results indicate that the structure (composition) of the mesophotic communities varied predominantly with seasons. We analyzed further the better-resolved fish assemblage and compared eDNA with other visual methods at the same locations and depths. We recovered eDNA from 19 genera of fish, six of these have also been observed on towed underwater videos, while 13 were unique to eDNA. We discuss the potential drivers of seasonal differences in community composition and richness. Our results suggest that eDNA can provide valuable insights for monitoring TMRE communities but highlight the necessity of completing reference DNA databases available for this region.pt_PT
dc.description.sponsorshipGrant number ICM_ NCN19_056pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/ece3.10999pt_PT
dc.identifier.issn2045-7758
dc.identifier.urihttp://hdl.handle.net/10400.1/20534
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiodiversity surveypt_PT
dc.subjecteDNApt_PT
dc.subjectEukaryotespt_PT
dc.subjectFishespt_PT
dc.subjectMetabarcodingpt_PT
dc.titleEnvironmental DNA reveals temporal variation in mesophotic reefs of the Humboldt upwelling ecosystems of central Chile: toward a baseline for biodiversity monitoring of unexplored marine habitatspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.titleEcology and Evolutionpt_PT
oaire.citation.volume14pt_PT
person.familyNameNavarro Campoy
person.givenNameAna
person.identifier2728884
person.identifier.ciencia-id7717-7301-3295
person.identifier.orcid0000-0002-6434-4326
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4f643b24-b9ff-42f4-b7e3-1c5f770ab65b
relation.isAuthorOfPublication.latestForDiscovery4f643b24-b9ff-42f4-b7e3-1c5f770ab65b

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