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Antibacterial activity of PDDA-stabilized GO-AgNP nanocomposites

2026-05-27Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorSadoq, Badr-Edine
dc.contributor.authorElamine, Youssef
dc.contributor.authorLeal, Inês
dc.contributor.authorBouajaj, Adel
dc.contributor.authorBritel, Mohammed Reda
dc.contributor.authorMaurady, Amal
dc.contributor.authorPower, Deborah Mary
dc.date.accessioned2026-06-30T09:06:57Z
dc.date.available2026-06-30T09:06:57Z
dc.date.issued2026-05-27
dc.description.abstractGraphene oxide (GO)-silver nanoparticle (AgNP) nanocomposites are widely studied for their antimicrobial synergy. In this study, the antibacterial activity of GO– AgNP nanocomposites prepared with poly(diallyldimethylammonium chloride) (PDDA) as a stabilizing linker was investigated. The composites were characterized by UV–Vis spectroscopy, FTIR, zeta potential analysis, XRD, TGA, and MPAES. GO–PDDA–AgNPs exhibited a strong surface plasmon resonance band at ~ 420 nm, new C–H stretching bands at 2865–3012 cm⁻¹ from PDDA, and a high positive zeta potential (+ 57.5 mV) compared to bare GO (–40 mV), confirming successful functionalization and improved colloidal stability. Antibacterial activity was evaluated against E. coli and S. aureus using disk diffusion assays, growth curves, and MIC determination. GO and AgNPs alone showed no inhibition at concentrations up to 20 µg/mL, whereas PDDA alone produced inhibition zones of 6–11.5 mm against S. aureus (2.5–100 µg/mL) and induced selective bacterial aggregation. The minimum inhibitory concentration (MIC) of PDDA was between 6.25 and 12.5 µg/mL for S. aureus and between 25 and 50 µg/mL for E. coli, indicating greater potency toward Gram-positive bacteria. The GO–PDDA–AgNP composite inhibited S. aureus with zones of 8.5–10 mm at 10–20 µg/mL, while no significant inhibition was observed for E. coli at the tested concentrations. Molecular docking simulations examining interactions between PDDA and quorum-sensing regulatory proteins AgrA in S. aureus and LsrR in E. coli predicted higher binding affinity to AgrA (–5.11 kcal/mol) than LsrR (–3.76 kcal/mol). While in vitro assays using a Chromobacterium violaceum CV026 biosensor showed no inhibition of AHL-mediated signalling, it should be noted that this model differs mechanistically from the Gram-positive agr system, leaving the predicted AgrA interaction as a potential target for future investigation. Ultimately, this study demonstrated that PDDA is the primary antibacterial component of the GO–PDDA–AgNP composite, exhibiting potent activity against Gram-positive bacteria through a mechanism involving selective bacterial aggregation.eng
dc.description.sponsorshipEMBRC.PT ALG-01-0145-FEDER-022121
dc.identifier.doi10.1007/s00289-026-06477-4
dc.identifier.eissn1436-2449
dc.identifier.issn0170-0839
dc.identifier.urihttp://hdl.handle.net/10400.1/29168
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofPolymer Bulletin
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGO-AgNP composites
dc.subjectPolymeric stabilizer
dc.subjectFunctional nanomaterials
dc.titleAntibacterial activity of PDDA-stabilized GO-AgNP nanocompositeseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/04326/2020
oaire.awardNumberUIDP/04326/2020
oaire.awardNumberLA/P/0101/2020
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue8
oaire.citation.startPage441
oaire.citation.titlePolymer Bulletin
oaire.citation.volume83
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLeal
person.familyNamePower
person.givenNameInês
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0009-0003-2522-4553
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication756b552d-df36-496b-b623-12476a58962f
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
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