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Characterization of ten highly polymorphic microsatellite loci for the intertidal mussel Perna perna, and cross species amplification within the genus

2012-10-08Journal article Open access
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dc.contributor.authorCoelho, Nelson
dc.contributor.authorI Zardi, Gerardo
dc.contributor.authorPearson, G. A.
dc.contributor.authorSerrão, Ester
dc.contributor.authorNicastro, Katy R
dc.date.accessioned2013-01-15T15:49:11Z
dc.date.available2013-01-15T15:49:11Z
dc.date.issued2012-10-08
dc.date.updated2012-12-12T20:09:35Z
dc.description.abstractThe brown mussel Perna perna (Linnaeus, 1758) is a dominant constituent of intertidal communities and a strong invader with multiple non-native populations distributed around the world. In a previous study, two polymorphic microsatellite loci were developed and used to determine population-level genetic diversity in invasive and native P. perna populations. However, higher number of microsatellite markers are required for reliable population genetic studies. In this context, in order to understand P. perna origins and history of invasion and to compare population genetic structure in native versus invaded areas, we developed 10 polymorphic microsatellite markers. Findings Described microsatellite markers were developed from an enriched genomic library. Analyses and characterization of loci using 20 individuals from a population in Western Sahara revealed on average 11 alleles per locus (range: 5–27) and mean gene diversity of 0.75 (range: 0.31 - 0.95). One primer pair revealed possible linkage disequilibrium while heterozygote deficiency was significant at four loci. Six of these markers cross-amplified in P. canaliculus (origin: New Zealand). Conclusions Developed markers will be useful in addressing a variety of questions concerning P. perna, including dispersal scales, genetic variation and population structure, in both native and invaded areas.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationBMC Research Notes. 2012 Oct 08;5(1):558
dc.identifier.doihttp://dx.doi.org/10.1186/1756-0500-5-558
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/2083
dc.language.isoengeng
dc.language.rfc3066eng
dc.peerreviewedyeseng
dc.rights.holderNelson C Coelho et al.; licensee BioMed Central Ltd.
dc.titleCharacterization of ten highly polymorphic microsatellite loci for the intertidal mussel Perna perna, and cross species amplification within the genus
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BEC%2F103916%2F2008/PT
oaire.citation.issue1:558por
oaire.citation.titleBMC Research Noteseng
oaire.citation.volume5eng
oaire.fundingStream3599-PPCDT
person.familyNameCoelho
person.familyNameZardi
person.familyNamePearson
person.familyNameSerrao
person.familyNameNicastro
person.givenNameNelson
person.givenNameGerardo
person.givenNameGareth Anthony
person.givenNameEster A.
person.givenNameKaty
person.identifier113536
person.identifierC-6686-2012
person.identifier.ciencia-id3113-ECA6-BF96
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person.identifier.orcid0000-0002-8331-5889
person.identifier.orcid0000-0001-8798-5794
person.identifier.orcid0000-0002-0768-464X
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0002-7071-141X
person.identifier.ridM-4026-2013
person.identifier.ridJ-3911-2013
person.identifier.ridD-2259-2012
person.identifier.scopus-author-id26029686700
person.identifier.scopus-author-id12647766300
person.identifier.scopus-author-id55916875600
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id12646675100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticle
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