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The regulatory action of estrogen and vasoactive intestinal peptide on prolactin secretion in sea bream (Sparus aurata, L.)

2003Journal article Restricted access
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dc.contributor.authorBrinca, Lilia
dc.contributor.authorFuentes, J.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T10:50:02Z
dc.date.available2014-10-24T10:50:02Z
dc.date.issued2003
dc.description.abstractThe effect of estradiol-17b (E2) implants on the in vitro secretion of prolactin (PRL) and its modulation by vasoactive intestinal peptide (VIP) in a marine teleost, sea bream (Sparus aurata L.), was determined. Experiments were conducted during winter and spring. During winter, fish (n¼130, body weight 50–70 g) were randomly divided into 2 groups; control and E2 treated (10 mg/kg, wet weight). Fish were sacrificed after 7 days treatment and in vitro pituitary cultures in Ringer bicarbonate supplemented with increasing doses (0–200 nM) of VIP were carried out for 18 h. Culture medium was analysed by PAGE and secreted PRL quantified by densitometry. Fish treated with E2 secreted significantly more PRL ðP < 0:05Þ in vitro than control fish. In E2 primed fish VIP caused a dose-dependent inhibition of PRL secretion in vitro. VIP had no detectable effect on the secretion of PRL from control pituitaries. Treatment with E2 had a different effect during spring; PRL secretion was significantly decreased ðP < 0:01Þ compared with the control fish. Anatomical evidence of abundant VIPimmunoreactive nerve fibres in neurohypophysial (NH) tissue penetrating the rostral pars distalis provide further evidence supporting an action for VIPin the regulation of PRL cells. In conclusion, the responsiveness of PRL in the pituitary gland varied with season. Moreover, in the sea bream VIP appears to modulate PRL secretion from E2 primed pituitary glands.por
dc.description.sponsorshipThe present work was funded by the Fundac ~ao para a Ci^encia e a Tecnologia, Portugal (PraxisXXI 2/2.1/ BIA/211/94). J.F. and L.B. were funded by FCT Grants BPD/22033 and 4/5.3/PRODEP/2000, respectively. The authors thank the anonymous reviewers for their helpful critique.por
dc.identifier.citationLilia Brinca, Juan Fuentes, and D.M. Power, "The regulatory action of estrogen and vasoactive intestinal peptide on prolactin secretion in sea bream (Sparus aurata, L.)" in General and Comparative Endocrinology 131 (2003) 117–125.por
dc.identifier.doihttp://dx.doi.org/10.1016/S0016-6480(02)00628-7
dc.identifier.issn0016-6480
dc.identifier.otherAUT: LBR00503; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5469
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectEstrogenpor
dc.subjectProlactinpor
dc.subjectRegulationpor
dc.subjectSeasonpor
dc.subjectVasoactive intestinal peptidepor
dc.subjectTeleost fishpor
dc.titleThe regulatory action of estrogen and vasoactive intestinal peptide on prolactin secretion in sea bream (Sparus aurata, L.)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage125por
oaire.citation.startPage117por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume131por
person.familyNameBrinca
person.familyNameFuentes
person.familyNamePower
person.givenNameLilia
person.givenNameJuan
person.givenNameDeborah Mary
person.identifier392998
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9135-8646
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicatione79f75f7-ccb6-46d0-a7d6-54bf8afbefaa
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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