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Prevalence and diagnostic comparison of helicobacter pylori and non-helicobacter pylori helicobacter infections in patients undergoing upper gastrointestinal endoscopy with gastric biopsy in Algarve, Portugal

2025-07-17Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg04:Educação de Qualidade
dc.contributor.authorMateus, Teresa Letra
dc.contributor.authorAguieiras, Catarina
dc.contributor.authorLouro, Ricardo
dc.contributor.authorPeixe, Bruno
dc.contributor.authorCalhindro, Mauro
dc.contributor.authorNunes, Francisco José Viegas Cortez
dc.contributor.authorQueirós, Patrícia
dc.contributor.authorCastelo-Branco, Pedro
dc.date.accessioned2026-04-09T12:56:29Z
dc.date.available2026-04-09T12:56:29Z
dc.date.issued2025-07-17
dc.description.abstractH. pylori infects over half of the global population and is associated with various gastric and extra-gastric diseases. Other species, such as zoonotic non-Helicobacter pylori Helicobacters (NHPHs), have shown similar associations with gastritis and MALT lymphoma and H. pylori-negative cases with gastric disease have been identified, including gastric MALT lymphoma, chronic gastritis, and gastroduodenal ulcers. Accurate identification of these species is of great relevance but remains challenging using conventional diagnostic methods. This cross-sectional study aimed to determine the prevalence of H. pylori and NHPH infections, comparing standard histological protocols with molecular techniques. Between December 2024 and February 2025, 54 adult patients undergoing upper gastrointestinal endoscopy (UGE) with gastric biopsy in three hospitals in Algarve, Portugal were recruited. Endoscopic assessment was performed, and gastric biopsies were collected for histological and molecular analysis. DNA was extracted from antral biopsies and analyzed by conventional PCR to detect H. pylori and NHPH. H. pylori diagnostic techniques were compared, descriptive plus statistical analysis was performed, and p-values < 0.05 were considered to be statistically significant. Fifty-four patients were included in the study, with 51.9% of them presenting symptoms. Endoscopic gastritis was observed in 66.7% of patients, while histological gastritis was present in 88.9%, with statistically significant differences between the two diagnostic techniques (p = 0.004). Helicobacter spp. were identified in 44.4% (24/54) of the patients. H. pylori was detected in 42.6% of the patients by Modified Giemsa stain and in 33.3% by PCR. H. bizzozeronii was found in 35.9% of the patients, with 22.2% showing mixed infections. This study reveals a significant prevalence of Helicobacter spp. in patients from the Algarve region, with both H. pylori and zoonotic H. bizzozeronii detected. This is the first report of H. bizzozeronii DNA detection in gastric biopsies via PCR from patients undergoing UGE in Portugal, highlighting the need to consider NHPH in clinical diagnosis. It is important to include molecular methods in routine diagnostics and the need for broader studies to assess regional and national trends in Helicobacter infections besides H. pylori.eng
dc.identifier.doi10.3390/microorganisms13071684
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.1/28634
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationCenter for Research and Development in Agrifood Systems and Sustainability
dc.relationCenter for Research and Development in Agrifood Systems and Sustainability
dc.relation.ispartofMicroorganisms
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGastritis
dc.subjectNHPH
dc.subjectHelicobacter bizzozeronii
dc.subjectModified giemsa
dc.subjectPCR
dc.titlePrevalence and diagnostic comparison of helicobacter pylori and non-helicobacter pylori helicobacter infections in patients undergoing upper gastrointestinal endoscopy with gastric biopsy in Algarve, Portugaleng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/05937/2020
oaire.awardNumberUIDP/05937/2020
oaire.awardTitleCenter for Research and Development in Agrifood Systems and Sustainability
oaire.awardTitleCenter for Research and Development in Agrifood Systems and Sustainability
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05937%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05937%2F2020/PT
oaire.citation.issue7
oaire.citation.startPage1684
oaire.citation.titleMicroorganisms
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameNunes
person.familyNameQueirós
person.familyNameCastelo-Branco
person.givenNameFrancisco José Viegas Cortez
person.givenNamePatrícia
person.givenNamePedro
person.identifier.ciencia-idE015-7F8F-5CA1
person.identifier.orcid0000-0001-6638-9782
person.identifier.orcid0000-0003-3575-426X
person.identifier.orcid0000-0002-3453-3978
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationaf03f692-441f-4403-964b-598270e28615
relation.isAuthorOfPublication956d1326-eaa7-4251-a987-e010449a8c20
relation.isAuthorOfPublicationbb25b5ad-1769-42be-a7d3-8fe76215aa23
relation.isAuthorOfPublication.latestForDiscoveryaf03f692-441f-4403-964b-598270e28615
relation.isProjectOfPublication61f7a7ad-cc0a-49ae-99c6-f46b1341a1b6
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relation.isProjectOfPublication.latestForDiscovery61f7a7ad-cc0a-49ae-99c6-f46b1341a1b6

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