Repository logo
 
Publication

Expression and trans-specific polymorphism of self-incompatibility RNases in Coffea (Rubiaceae)

2011-06Journal article Open access
Simple item page
dc.contributor.authorNowak, Michael D.
dc.contributor.authorDavis, Aaron P.
dc.contributor.authorAnthony, Francois
dc.contributor.authorYoder, Anne D.
dc.date.accessioned2018-12-07T14:53:33Z
dc.date.available2018-12-07T14:53:33Z
dc.date.issued2011-06
dc.description.abstractSelf-incompatibility (SI) is widespread in the angiosperms, but identifying the biochemical components of SI mechanisms has proven to be difficult in most lineages. Coffea (coffee; Rubiaceae) is a genus of old-world tropical understory trees in which the vast majority of diploid species utilize a mechanism of gametophytic self-incompatibility (GSI). The S-RNase GSI system was one of the first SI mechanisms to be biochemically characterized, and likely represents the ancestral Eudicot condition as evidenced by its functional characterization in both asterid (Solanaceae, Plantaginaceae) and rosid (Rosaceae) lineages. The S-RNase GSI mechanism employs the activity of class III RNase T2 proteins to terminate the growth of "self" pollen tubes. Here, we investigate the mechanism of Coffea GSI and specifically examine the potential for homology to S-RNase GSI by sequencing class III RNase T2 genes in populations of 14 African and Madagascan Coffea species and the closely related self-compatible species Psilanthus ebracteolatus. Phylogenetic analyses of these sequences aligned to a diverse sample of plant RNase T2 genes show that the Coffea genome contains at least three class III RNase T2 genes. Patterns of tissue-specific gene expression identify one of these RNase T2 genes as the putative Coffea S-RNase gene. We show that populations of SI Coffea are remarkably polymorphic for putative S-RNase alleles, and exhibit a persistent pattern of trans-specific polymorphism characteristic of all S-RNase genes previously isolated from GSI Eudicot lineages. We thus conclude that Coffea GSI is most likely homologous to the classic Eudicot S-RNase system, which was retained since the divergence of the Rubiaceae lineage from an ancient SI Eudicot ancestor, nearly 90 million years ago.
dc.description.sponsorshipUnited States National Science Foundation [0849186]; Society of Systematic Biologists; American Society of Plant Taxonomists; Duke University Graduate School
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.pone.0021019
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/11568
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectF-box gene
dc.subjectInbreeding depression
dc.subjectNicotiana-Alata
dc.subjectEvolutionary consequences
dc.subjectRecent speciation
dc.subjectS-Locus
dc.subjectPollen
dc.subjectPlants
dc.subjectSolanaceae
dc.subjectRibonucleases
dc.titleExpression and trans-specific polymorphism of self-incompatibility RNases in Coffea (Rubiaceae)
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6
oaire.citation.startPagee37251
oaire.citation.titlePLoS ONE
oaire.citation.volume6
person.familyNameNowak
person.givenNameMichael
person.identifier.orcid0000-0001-8924-6506
person.identifier.ridM-3747-2017
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication56866820-89ba-4d03-a818-b211490c854e
relation.isAuthorOfPublication.latestForDiscovery56866820-89ba-4d03-a818-b211490c854e

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
handle11568.pdf
Size:
995.84 KB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)