Publication
Immobilization of spray dried E. coli containing recombinant aminotransferase using hydrous titanium oxide
| dc.contributor.advisor | Adlercreutz, Patrick | |
| dc.contributor.advisor | Grey, Carl | |
| dc.contributor.advisor | Barros, Raúl José Jorge de | |
| dc.contributor.author | Neto, Watson | |
| dc.date.accessioned | 2011-09-07T16:05:41Z | |
| dc.date.available | 2011-09-07T16:05:41Z | |
| dc.date.issued | 2009 | |
| dc.description | Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2009 | por |
| dc.description.abstract | Chiral amines are important building blocks for the pharmaceutical and agrochemical industry. They have many applications and are therefore of economical importance. Their production has mostly been achieved by enzymatic resolution. Aminotransferase, the enzyme most commonly used is known to be a pyridoxal-5- phosphate (PLP) dependent enzyme and it is commercialized in a spray dried Escherichia coli formulation. Since there is a great market for this enzyme and all the products it can synthesize, it is essential to constantly improve its efficiency and make it even more economically attractive. Several studies have been conducted to achieve this purpose, one of these being the reutilization of the biocatalyst by immobilization. In this work, immobilization of the biocatalyst was performed using a different approach than what has been tried before. The approach is based on interaction between hydrous transition metal oxides, mainly the hydrous titanium oxide (TiOx), and biological entities such as cells, enzymes and similar. Hydrous titanium oxide has the ability to bind the cells and enzymes via hydroxyl groups forming partial covalent bonds. Using this method, the biocatalyst was successfully immobilized and its reutilization was demonstrated for several batches. Studies on enzyme kinetics, storage stability, temperature effect, and loading capacity were performed to validate the usefulness of the immobilization method for the potential use in a large scale process. | eng |
| dc.format | application/pdf | por |
| dc.identifier.other | 577 NET*Imm Cave | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/832 | |
| dc.language.iso | eng | por |
| dc.subject | Teses | por |
| dc.subject | Enzimas | por |
| dc.subject | Óxido de titânio hidratado | por |
| dc.subject | Transaminase | por |
| dc.title | Immobilization of spray dried E. coli containing recombinant aminotransferase using hydrous titanium oxide | por |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | por |
| rcaap.type | masterThesis | por |