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Phenols, flavonoids and antioxidant activity of aqueous and methanolic extracts of propolis (Apis mellifera L.) from Algarve, South Portugal

2014Journal article Restricted access
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dc.contributor.authorMiguel, Maria Graça
dc.contributor.authorNunes, S.
dc.contributor.authorDandlen, S. A
dc.contributor.authorCavaco, A. M.
dc.contributor.authorAntunes, Maria Dulce
dc.date.accessioned2015-06-15T14:53:22Z
dc.date.available2015-06-15T14:53:22Z
dc.date.issued2014
dc.description.abstractPropolis is a resinous substance collected by honeybees to seal honeycomb, which has been used in folk medicine due to its antimicrobial and antioxidant properties. In the present study, water and methanol were used to extract phenols and flavonoids from propolis collected in thirteen different areas in the Algarve region during the winter and spring. The ABTS(center dot+), DPPH center dot, and O-2(center dot-) scavenging capacity, and metal chelating activity were also evaluated in the propolis samples. Methanol was more effective than water in extracting total phenols (2.93-8.76 mg/mL) (0.93-2.81 mg/mL). Flavones and flavonols were also better extracted with methanol (1.28-2.76 mg/mL) than with water (0.031-0.019 mg/mL). The free radical scavenging activity, ABTS (IC50 = 0.006-0.036 mg/mL), DPPH (IC50 = 0.007-0.069 mg/mL) and superoxide (IC50 = 0.001-0.053 mg/mL), of the samples was also higher in methanolic extracts. The capacity for chelating metal ions was higher in aqueous extracts (41.11-82.35%) than in the methanolic ones (4.33-29.68%). Propolis from three locations of Algarve region were richer in phenols and had better capacity for scavenging free ABTS and DPPH radicals than the remaining samples. These places are part of a specific zone of Algarve known as Barrocal.
dc.identifier.issn0101-2061
dc.identifier.issn1678-457X
dc.identifier.otherAUT: MGM00322; MAN00114;
dc.identifier.urihttp://hdl.handle.net/10400.1/6257
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSociedade Brasileira Ciência Tecnologia dos Alimentos
dc.relation.isbasedonP-009-RB0
dc.titlePhenols, flavonoids and antioxidant activity of aqueous and methanolic extracts of propolis (Apis mellifera L.) from Algarve, South Portugal
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage23
oaire.citation.startPage16
oaire.citation.titleFood Science and Technology
oaire.citation.volume34
person.familyNameMiguel
person.familyNameCavaco Guerra
person.familyNameAntunes
person.givenNameMaria da Graça
person.givenNameAna Margarida
person.givenNameMaria Dulce
person.identifierC-1285-2012
person.identifier177556
person.identifier.ciencia-id6110-6FE7-DF28
person.identifier.ciencia-idC91E-B434-E327
person.identifier.ciencia-idC11B-9B05-217E
person.identifier.orcid0000-0003-2507-4228
person.identifier.orcid0000-0003-2708-5991
person.identifier.orcid0000-0002-8913-6136
person.identifier.ridA-4683-2012
person.identifier.scopus-author-id35562054500
person.identifier.scopus-author-id6602899707
person.identifier.scopus-author-id7102645075
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication2507350f-9e60-4d4a-a096-53e58d93549c
relation.isAuthorOfPublication18ad736f-b1f3-4a19-9636-e7a80248cf94
relation.isAuthorOfPublication7947cc50-4ae0-4ada-8ddf-081f247adc90
relation.isAuthorOfPublication.latestForDiscovery18ad736f-b1f3-4a19-9636-e7a80248cf94

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