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Detection of novel potential regulators of stem cell differentiation and Cardiogenesis through Combined Genome-Wide profiling of Protein-Coding transcripts and microRNAs

2021-09Journal article Open access
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dc.contributor.authorMachado, Rui
dc.contributor.authorSachinidis, Agapios
dc.contributor.authorFutschik, Matthias
dc.date.accessioned2021-10-06T13:23:28Z
dc.date.available2021-10-06T13:23:28Z
dc.date.issued2021-09
dc.description.abstractIn vitro differentiation of embryonic stem cells (ESCs) provides a convenient basis for the study of microRNA-based gene regulation that is relevant for early cardiogenic processes. However, to which degree insights gained from in vitro differentiation models can be readily transferred to the in vivo system remains unclear. In this study, we profiled simultaneous genome-wide measurements of mRNAs and microRNAs (miRNAs) of differentiating murine ESCs (mESCs) and integrated putative miRNA-gene interactions to assess miRNA-driven gene regulation. To identify interactions conserved between in vivo and in vitro, we combined our analysis with a recent transcriptomic study of early murine heart development in vivo. We detected over 200 putative miRNA–mRNA interactions with conserved expression patterns that were indicative of gene regulation across the in vitro and in vivo studies. A substantial proportion of candidate interactions have been already linked to cardiogenesis, supporting the validity of our approach. Notably, we also detected miRNAs with expression patterns that closely resembled those of key developmental transcription factors. The approach taken in this study enabled the identification of miRNA interactions in in vitro models with potential relevance for early cardiogenic development. Such comparative approaches will be important for the faithful application of stem cells in cardiovascular research.pt_PT
dc.description.sponsorshipSA 568/17-2
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/cells10092477pt_PT
dc.identifier.eissn2073-4409
dc.identifier.urihttp://hdl.handle.net/10400.1/17194
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation57213647pt_PT
dc.relationCentre of Marine Sciences
dc.relationDeciphering the gene regulatory networks of cyanobacteria
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectStem cell differentiationpt_PT
dc.subjectGene regulationpt_PT
dc.subjectMicroRNAspt_PT
dc.subjectTranscriptomicspt_PT
dc.subjectCardiogenesispt_PT
dc.titleDetection of novel potential regulators of stem cell differentiation and Cardiogenesis through Combined Genome-Wide profiling of Protein-Coding transcripts and microRNAspt_PT
dc.title.alternativeDetecção de novos reguladores potenciais de diferenciação de células-tronco e cardiogênese através de perfil combinado em todo o genoma de transcrições de codificação de proteínas e microRNAspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleDeciphering the gene regulatory networks of cyanobacteria
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PD/PD%2FBD%2F105894%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00881%2F2013%2FCP1198%2FCT0001/PT
oaire.citation.issue9pt_PT
oaire.citation.startPage2477pt_PT
oaire.citation.titleCellspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamPD
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamInvestigador FCT
person.familyNameMachado
person.familyNameFutschik
person.givenNameRui
person.givenNameMatthias
person.identifier.ciencia-id5D19-8F5F-3CE1
person.identifier.ciencia-idA71B-AD01-3501
person.identifier.orcid0000-0002-4080-2353
person.identifier.orcid0000-0002-6245-8071
person.identifier.scopus-author-id55991033900
person.identifier.scopus-author-id14017989400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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