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Activation profile of the Atlantic salmon (Salmo salar) calcium-sensing receptor (Casr) by selected L-amino acids

2025-04-17Journal article Open access
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dc.contributor.authorGomes, Ana
dc.contributor.authorGélébart, Virginie
dc.contributor.authorFélix, Rute
dc.contributor.authorCardoso, João
dc.contributor.authorZimmermann, Fabian
dc.contributor.authorLai, Floriana
dc.contributor.authorPower, Deborah Mary
dc.contributor.authorRonnestad, Ivar
dc.date.accessioned2025-05-26T12:28:57Z
dc.date.available2025-05-26T12:28:57Z
dc.date.issued2025-04-17
dc.description.abstractIn mammals, the calcium-sensing receptor (CaSR) is involved in nutrient sensing and modulated by several amino acids. In teleosts, sequence homologues of the mammalian CaSR have been described but their function in sensing amino acids remains elusive, including in Atlantic salmon (Salmo salar), an important aquaculture species. This study investigated the activation of Atlantic salmon Casr (asCasr)-mediated signaling pathways-Gq, Gi, and ERK1/2-by six selected L-amino acids (histidine, tryptophan, phenylalanine, isoleucine, leucine and valine) and by Ca2+. Using a Flp-In-HEK293 cell line stably expressing asCasr, we confirmed activation of all three pathways. L-histidine, L-phenylalanine, and L-tryptophan triggered Gi signaling independent of Ca-2(+). Notably, no Ca-2(+) concentrations induced Gi activation, but IP1 production increased in a concentration-dependent manner. L-histidine was the only amino acid to activate the Gq pathway without Ca-2(+), and this response was amplified by the presence of Ca-2(+). In the presence of 2.5 mM Ca-2(+), L-phenylalanine and L-tryptophan also activated Gq signaling in a concentration-dependent manner. Additionally, in the presence of 10 mM Ca-2(+), L-histidine, L-phenylalanine, and L-tryptophan triggered ERK phosphorylation. These findings establish asCasr as a functional homologue of mammalian CaSR, activated in a concentration-dependent manner by L-amino acids with an aromatic ring.eng
dc.identifier.doi10.1038/s41598-025-97483-5
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/27169
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofScientific Reports
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSignaling pathway
dc.subjectG(i)
dc.subjectG(q)
dc.subjectERK
dc.subjectNutrient sensing
dc.subjectTeleost
dc.titleActivation profile of the Atlantic salmon (Salmo salar) calcium-sensing receptor (Casr) by selected L-amino acidseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue1
oaire.citation.startPage13236
oaire.citation.titleScientific Reports
oaire.citation.volume15
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGomes
person.familyNameFélix
person.familyNameCardoso
person.familyNamePower
person.familyNameRonnestad
person.givenNameAna
person.givenNameRute
person.givenNameJoão
person.givenNameDeborah Mary
person.givenNameIvar
person.identifierR-000-75E
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-id5C1C-8247-D614
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-6565-9370
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0002-7353-1799
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0001-8789-0197
person.identifier.ridM-6164-2013
person.identifier.ridC-5552-2008
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id9639597300
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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