Publication
Variability of a portion of RNA 3 containing the coat protein gene of Citrus variegation virus (CVV) using single-strand conformation polymorphism (SSCP)
| dc.contributor.author | Bennani, B. | |
| dc.contributor.author | Zemzami, M. | |
| dc.contributor.author | Nolasco, Gustavo | |
| dc.contributor.author | Azeddoug, H. | |
| dc.date.accessioned | 2015-06-15T14:13:43Z | |
| dc.date.available | 2015-06-15T14:13:43Z | |
| dc.date.issued | 2002 | |
| dc.description.abstract | A portion of RNA 3 of Citrus variegation virus (CVV), comprising part of the intergenic region and the coat protein (CP) gene from eight viral isolates, was amplified by RT-PCR and cloned. The clones were compared for intra and inter-isolate variations by single-strand conformation polymorphism analysis. Some of the results were compared with sequence data previously obtained. The test discriminated between clones differing in as little as 3.2% of the nucleotides. Most isolates included several variants, in some cases with a predominant pattern, which, however, could no longer be recognised in new RT-PCR products obtained 13 months later. This procedure can there-fore be used to identify and detect variations between CVV isolates. It is rapid, inexpensive and may reduce the amount of sequencing needed for comparing viral isolates. | |
| dc.identifier.issn | 0031-9465 | |
| dc.identifier.other | AUT: GNO00324; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/6212 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.relation.isbasedon | P-007-WDM | |
| dc.title | Variability of a portion of RNA 3 containing the coat protein gene of Citrus variegation virus (CVV) using single-strand conformation polymorphism (SSCP) | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 192 | |
| oaire.citation.startPage | 188 | |
| oaire.citation.title | Phytopathologia Mediterranea | |
| oaire.citation.volume | 41 | |
| person.familyName | Nolasco | |
| person.givenName | Gustavo | |
| person.identifier.orcid | 0000-0002-7025-6023 | |
| person.identifier.scopus-author-id | 6602625528 | |
| rcaap.rights | openAccess | |
| rcaap.type | article | |
| relation.isAuthorOfPublication | 87a8035f-bc15-44dd-b8c3-4288c5660fff | |
| relation.isAuthorOfPublication.latestForDiscovery | 87a8035f-bc15-44dd-b8c3-4288c5660fff |
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