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Marine green macroalgae: a source of natural compounds with mineralogenic and antioxidant activities

2017-02Journal article Restricted access
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dc.contributor.authorSurget, Gwladys
dc.contributor.authorRoberto, Vania Palma
dc.contributor.authorLe Lann, Klervi
dc.contributor.authorMira, Sara
dc.contributor.authorGuerard, Fabienne
dc.contributor.authorLaizé, Vincent
dc.contributor.authorPoupart, Nathalie
dc.contributor.authorLeonor Cancela, M.
dc.contributor.authorStiger-Pouvreau, Valerie
dc.date.accessioned2019-11-20T15:07:49Z
dc.date.available2019-11-20T15:07:49Z
dc.date.issued2017-02
dc.description.abstractMarine macroalgae represent a valuable natural resource for bioactive phytochemicals with promising applications in therapeutics, although they remain largely under-exploited. In this work, the potential of two marine green macroalgae (Cladophora rupestris and Codium fragile) as a source of bioactive phenolic compounds was explored, and antioxidant, mineralogenic, and osteogenic activities were evaluated. For each species, a crude hydroalcoholic extract (CE) was prepared by solid/liquid extraction and fractionated by liquid/liquid purification into an ethyl acetate fraction (EAF) enriched in phenolic compounds and an aqueous fraction (AF). Antioxidant activity, assessed through radical scavenging activity and reducing power assay, was increased in EAF fraction of both species and closely related to the phenolic content in each fraction. Mineralogenic activity, assessed through extracellular matrix mineralization of a fish bone-derived cell line, was induced by EAF fractions (up to 600 % for C. rupestris EAF). Quantitative analysis of operculum formation in zebrafish larvae stained with alizarin red S further confirmed the osteogenic potential of EAF fractions in vivo, with an increase of more than 1.5-fold for both C. fragile and C. rupestris fractions, similar to vitamin D (control). Our results demonstrated a positive correlation between phenolic fractions and biological activity, suggesting that phenolic compounds extracted from marine green macroalgae may represent promising molecules toward therapeutic applications in the field of bone biology.
dc.description.sponsorshipEuropean Regional Development Fund (ERDF)-Atlantic Area Programme through MARMED project [2011-1/164]
dc.description.sponsorshipEuropean Era-Net, Seas-Era program through the project INVASIVES [ANR-12-SEAS-0002-01]
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT) [UID/Multi/04326/2013]
dc.identifier.doi10.1007/s10811-016-0968-3
dc.identifier.issn0921-8971
dc.identifier.issn1573-5176
dc.identifier.urihttp://hdl.handle.net/10400.1/13231
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.subjectSargassum-muticum
dc.subjectBrown-algae
dc.subjectIn-vitro
dc.subjectOsteoblastic differentiation
dc.subjectPhenolic-compounds
dc.subjectOxidative stress
dc.subjectMc3T3-E1 cells
dc.subjectBone loss
dc.subjectPhlorotannins
dc.subjectZebrafish
dc.titleMarine green macroalgae: a source of natural compounds with mineralogenic and antioxidant activities
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPage584
oaire.citation.issue1
oaire.citation.startPage575
oaire.citation.titleJournal of Applied Phycology
oaire.citation.volume29
oaire.fundingStream5876
person.familyNameRoberto
person.familyNameMira
person.familyNameLaizé
person.familyNameCancela
person.givenNameVânia
person.givenNameSara
person.givenNameVincent
person.givenNameM. Leonor
person.identifier1230353
person.identifier.ciencia-id681E-40B7-331F
person.identifier.orcid0000-0002-8134-4045
person.identifier.orcid0000-0001-8346-4449
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id27467657800
person.identifier.scopus-author-id6603417249
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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