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Gender differences in protein expression after polystyrene nanoplastics exposure in mussels mytilus galloprovincialis

2025-10Artigo científico Acesso aberto
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datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg05:Igualdade de Género
dc.contributor.authorRibeiro Gonçalves, Joanna Melissa
dc.contributor.authorMendes, Vera M.
dc.contributor.authorManadas, Bruno
dc.contributor.authorBebianno, Maria
dc.date.accessioned2026-03-20T13:35:24Z
dc.date.available2026-03-20T13:35:24Z
dc.date.issued2025-10
dc.description.abstractPlastic pollution is a significant issue that the scientific community has been actively studying due to its harmful effects on aquatic ecosystems. Nanoplastics can pass through cellular barriers and enter the mussel’s bloodstream. More worryingly, they can also penetrate sperm cells and oocytes, potentially impacting their motility and resilience. Reproductive success drives a shift in population dynamics and plays a vital role in maintaining a healthy ecosystem. Proteomics helps detect protein changes caused by exposure to contaminants, such as nanoplastics, in marine organisms, providing deeper molecular-level insights into contamination-induced cellular pathways. Therefore, this study aimed to utilise a high-throughput proteomic approach to evaluate the impact of polystyrene nanoplastics (PS-NPs) on the gonads of male and female M. galloprovincialis, using a SWATH-MS analysis after 21 days of exposure to 10 μg/L of PS-NPs (50 nm). The accumulation of PS-NPs was also evaluated in male and female mussels. A comparison in protein expression of controls vs. those exposed in male and female mussel gonads and between males and females was evaluated. The findings indicate that PS-NP accumulation in male gonads alters protein expression more significantly than in females, interfering with protein synthesis, energy production, intracellular transport, and cellular homeostasis, and possibly impaired reproductive function. Female gonads exposed to PS-NPs revealed disruption in proteins associated with translation, RNA processing and signaling, ribosome biogenesis, cell cycle regulation, and stress response. Protein folding, lipid metabolism, and calcium signaling pathways were also affected, leading to oogenesis, meiotic progression, intracellular transport, and energy metabolism impairments. These disruptions ultimately impact reproductive success and cellular homeostasis, leading to a decline in biodiversity.eng
dc.description.sponsorshipFCT JPIOCEANS/0005/2020; ALG498 01-0145-FEDER-030922; UID/00350/2020; LA/P/0058/2020
dc.identifier.doi10.1016/j.scitotenv.2025.180181
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.1/28493
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationEmerging: Environmental Mixtures of Emerging contaminants Repercussions on Gonads and Impact on Next Generations
dc.relationAquatic Research Infrastructure Network
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationLA/P/0058/2020
dc.relation.ispartofScience of The Total Environment
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectNanoplastics
dc.subjectGonads
dc.subjectGender
dc.subjectMytilus galloprovincialis
dc.subjectProteomics
dc.subjectPolystyrene
dc.titleGender differences in protein expression after polystyrene nanoplastics exposure in mussels mytilus galloprovincialiseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUI/BD/150758/2020
oaire.awardNumberLA/P/0069/2020
oaire.awardNumberUIDB/04539/2020
oaire.awardNumberUIDP/04539/2020
oaire.awardTitleEmerging: Environmental Mixtures of Emerging contaminants Repercussions on Gonads and Impact on Next Generations
oaire.awardTitleAquatic Research Infrastructure Network
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F150758%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0069%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04539%2F2020/PT
oaire.citation.startPage180181
oaire.citation.titleScience of the Total Environment
oaire.citation.volume997
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRibeiro Gonçalves
person.familyNameBebianno
person.givenNameJoanna Melissa
person.givenNameMaria
person.identifier.ciencia-id2D1D-F33A-FB48
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0002-3242-1032
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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