dc.contributor.author	Churcher, Allison
dc.contributor.author	Pujolar, Jose M.
dc.contributor.author	Milan, Massimo
dc.contributor.author	Hubbard, Peter
dc.contributor.author	Martins, Rute S. T.
dc.contributor.author	L. Saraiva, João
dc.contributor.author	Huertas, Mar
dc.contributor.author	Bargelloni, Luca
dc.contributor.author	Patarnello, T.
dc.contributor.author	Marino, Ilaria A. M.
dc.contributor.author	Zane, Lorenzo
dc.contributor.author	Canario, Adelino V. M.
dc.date.accessioned	2014-12-31T14:03:32Z
dc.date.available	2014-12-31T14:03:32Z
dc.date.issued	2014-09-17
dc.date.updated	2014-09-23T14:35:26Z
dc.description.abstract	Background: The vertebrate brain plays a critical role in the regulation of sexual maturation and reproduction by integrating environmental information with developmental and endocrine status. The European eel Anguilla anguilla is an important species in which to better understand the neuroendocrine factors that control reproduction because it is an endangered species, has a complex life cycle that includes two extreme long distance migrations with both freshwater and seawater stages and because it occupies a key position within the teleost phylogeny. At present, mature eels have never been caught in the wild and little is known about most aspects of reproduction in A. anguilla. The goal of this study was to identify genes that may be involved in sexual maturation in experimentally matured eels. For this, we used microarrays to compare the gene expression profiles of sexually mature to immature males. Results: Using a false discovery rate of 0.05, a total of 1,497 differentially expressed genes were identified. Of this set, 991 were expressed at higher levels in brains (forebrain and midbrain) of mature males while 506 were expressed at lower levels relative to brains of immature males. The set of up-regulated genes includes genes involved in neuroendocrine processes, cell-cell signaling, neurogenesis and development. Interestingly, while genes involved in immune system function were down-regulated in the brains of mature males, changes in the expression levels of several receptors and channels were observed suggesting that some rewiring is occurring in the brain at sexual maturity. Conclusions: This study shows that the brains of eels undergo major changes at the molecular level at sexual maturity that may include re-organization at the cellular level. Here, we have defined a set of genes that help to understand the molecular mechanisms controlling reproduction in eels. Some of these genes have previously described functions while many others have roles that have yet to be characterized in a reproductive context. Since most of the genes examined here have orthologs in other vertebrates, the results of this study will contribute to the body of knowledge concerning reproduction in vertebrates as well as to an improved understanding of eel biology.	por
dc.description.version	Peer Reviewed
dc.identifier.citation	BMC Genomics. 2014 Sep 17;15(1):799	por
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2164-15-799
dc.identifier.uri	http://hdl.handle.net/10400.1/5694
dc.language.iso	eng	por
dc.peerreviewed	yes	por
dc.publisher	BioMed Central	por
dc.rights.holder	Allison M Churcher et al.; licensee BioMed Central Ltd.
dc.subject	Eel	por
dc.subject	Microarray	por
dc.subject	Neuroendocrine	por
dc.subject	Gene expression	por
dc.subject	Brain	por
dc.subject	Reproduction	por
dc.title	Changes in the gene expression profiles of the brains of male European eels (Anguilla anguilla) during sexual maturation	por
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	13	por
oaire.citation.issue	(1):799	por
oaire.citation.startPage	1	por
oaire.citation.title	BMC Genomics	por
oaire.citation.volume	15	por
person.familyName	Churcher
person.familyName	Hubbard
person.familyName	Saraiva
person.familyName	Huertas
person.familyName	Canario
person.givenName	Allison
person.givenName	Peter
person.givenName	João
person.givenName	Mar
person.givenName	Adelino
person.identifier	292979
person.identifier	143624
person.identifier.ciencia-id	951F-BA4B-A21F
person.identifier.ciencia-id	7A15-0E73-E652
person.identifier.ciencia-id	1F1E-D3B3-F804
person.identifier.orcid	0000-0003-1902-3002
person.identifier.orcid	0000-0002-3007-4647
person.identifier.orcid	0000-0002-8891-8881
person.identifier.orcid	0000-0002-8042-086X
person.identifier.orcid	0000-0002-6244-6468
person.identifier.rid	M-6169-2013
person.identifier.rid	B-2823-2008
person.identifier.rid	M-6167-2013
person.identifier.rid	B-2898-2008
person.identifier.rid	C-7942-2009
person.identifier.scopus-author-id	15062343200
person.identifier.scopus-author-id	7005928467
person.identifier.scopus-author-id	26325116400
person.identifier.scopus-author-id	56568523700
rcaap.rights	openAccess	por
rcaap.type	article	por
relation.isAuthorOfPublication	c7392254-eaa2-4cd6-86b2-d350ab7c8d82
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relation.isAuthorOfPublication.latestForDiscovery	5f6e51ee-9113-469e-8b9e-f30f2d452521

Changes in the gene expression profiles of the brains of male European eels (Anguilla anguilla) during sexual maturation

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