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The induction of DNA strand breaks at specific sites by N-nitroso-N-ethylurea depends on the phases of the cell cycle

1987Journal article Restricted access
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dc.contributor.authorLeitão, José
dc.contributor.authorPetkova, S.
dc.contributor.authorDjondjurov, L.
dc.date.accessioned2015-06-19T14:36:30Z
dc.date.available2015-06-19T14:36:30Z
dc.date.issued1987
dc.description.abstractSynchronized root meristems of Pisum sativum were treated at each phase of the cell cycle with 6.25 mM N-nitroso-N-ethylurea. DNA extracted from treated cells and run in agarose gel electrophoresis exhibits a series of discrete fragments with length below 2500 bp and a significant number of unspecific single-stranded breaks (or alkali-labile sites). Experiments with micrococcal nuclease indicated that the nucleosomal organization of the chromatin is not responsible for the generation of the discrete fragments: it seems that their appearance is associated with a preferable attack of the mutagen at specific sites, characteristic for the plant genome. Moreover, a cell cycle dependent release of the discrete fragments was found with maximum at G1-S and minimum at mitosis. The model experiments designed to clarify this observation suggest that it might be determined from the cell cycle dependent fluctuation in the accessibility of the chromatin DNA and/or the process of excision-repair.
dc.identifier.otherAUT: JLE00446
dc.identifier.urihttp://hdl.handle.net/10400.1/6539
dc.language.isoeng
dc.peerreviewedyes
dc.relation.isbasedonP-008-ZRK
dc.titleThe induction of DNA strand breaks at specific sites by N-nitroso-N-ethylurea depends on the phases of the cell cycle
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage248
oaire.citation.startPage239
oaire.citation.titleMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
oaire.citation.volume180
person.familyNameLeitão
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery0f990d1c-2913-4f8a-8fb6-f8b02721247f

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