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Heterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: Medium formulation and process scale-up

2025-04Journal article Restricted access
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dc.contributor.authorTrovão, Mafalda
dc.contributor.authorBarros, Ana
dc.contributor.authorMachado, Adriana
dc.contributor.authorReis, Ana
dc.contributor.authorPedroso, Humberto
dc.contributor.authorEspírito Santo, Gonçalo
dc.contributor.authorCorreia, Nádia
dc.contributor.authorCosta, Monya
dc.contributor.authorFerreira, Sara
dc.contributor.authorVarela, João
dc.contributor.authorCardoso, Helena
dc.contributor.authorSilva, Joana
dc.contributor.authorPereira, Hugo
dc.contributor.authorFreitas, Filomena
dc.date.accessioned2025-02-07T11:19:28Z
dc.date.available2025-02-07T11:19:28Z
dc.date.issued2025-04
dc.description.abstractMicroalgal protein is a promising feedstock to complement and/or replace other protein sources. Besides requiring less land and water usage, microalgae production is a more sustainable process, especially if industrial sidestreams are used as nutrient sources. Additionally, the heterotrophic cultivation of microalgae, such as Chlorella vulgaris, enables the achievement of much higher biomass productivity and lower areal footprint than autotrophic cultivation. Chlorophyll-deficient strains of C. vulgaris, as the yellow strain 7Y, provide microalgal biomass with improved sensory properties. In line with this, a waste-based medium was formulated to cultivate this strain, aiming at maximum biomass productivity. In this context, several industrial sidestreams were screened, and two food wastes and corn molasses were selected for their high nitrogen and glucose concentrations, respectively. The waste-based medium formulated was compared to the inorganic optimised medium at laboratory scale in Erlenmeyer flasks and 7-L reactors. The results obtained in the 7-L fermenters revealed that both conditions achieved similar biomass productivities and growth rates of approximately 14 g L-1 d-1 and 0.8 d-1, respectively. The biomass and protein productivities were further enhanced by supplying a higher nitrogen concentration in the feeding solution when the process was scaled-up to 200-L reactors, reaching 22 and 6.3 g L-1 d-1, respectively, thus validating the developed industrial waste-based medium for the efficient cultivation of C. vulgaris under heterotrophic conditions.eng
dc.description.sponsorship2020.06814.BD PhD; 2020.07229.BD PhD; 2020.06814.BDANA
dc.identifier.doi10.1016/j.jece.2025.115361
dc.identifier.issn2213-3437
dc.identifier.urihttp://hdl.handle.net/10400.1/26761
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationSystemic Innovations Towards a Zero Food Waste Supply Chain
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation2020.06814.BDANA
dc.relation.ispartofJournal of Environmental Chemical Engineering
dc.rights.uriN/A
dc.subjectBiomass productivity
dc.subjectFood waste
dc.subjectHeterotrophic cultivation
dc.subjectSidestreams
dc.subjectMicroalgae
dc.subjectProtein productivity
dc.titleHeterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: Medium formulation and process scale-upeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSystemic Innovations Towards a Zero Food Waste Supply Chain
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/101036388/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue2
oaire.citation.startPage115361
oaire.citation.titleJournal of Environmental Chemical Engineering
oaire.citation.volume13
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameVarela
person.givenNameJoão
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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