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Pericentromeric location of the telomeric DNA sequences on the European grayling chromosomes

2013-12Journal article Open access
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dc.contributor.authorOcalewicz, K.
dc.contributor.authorFurgala-Selezniow, G.
dc.contributor.authorSzmyt, M.
dc.contributor.authorLisboa, R.
dc.contributor.authorKucinski, M.
dc.contributor.authorLejk, A. M.
dc.contributor.authorJankun, Malgorzata
dc.date.accessioned2018-12-07T14:52:49Z
dc.date.available2018-12-07T14:52:49Z
dc.date.issued2013-12
dc.description.abstractThe chromosomal characteristics, locations and variations of the C-band positive heterochromatin and telomeric DNA sequences were studied in the European grayling karyotype (Thymallus thymallus, Salmonidae) using conventional C-banding, endonucleases digestion banding, silver nitrate (AgNO3), chromomycin A(3) and 4',6-diamidino-2-phenylindole staining techniques as well as fluorescence in situ hybridization (FISH) and primed in situ labelling. Original data on the chromosomal distribution of segments resistant to AluI restriction endonuclease and identification of the C-banded heterochromatin presented here have been used to characterize the grayling karyotype polymorphism. Structural and length polymorphism of the chromosome 21 showing a conspicuous heterochromatin block adjacent to the centromere seems to be the result of the deletion and inversion. Two pairs of nuclear organizer regions (NOR)-bearing chromosomes were found to be polymorphic in size and displaying several distinct forms. FISH with telomeric peptide nucleic acid probe enabled recognition of the conservative telomeric DNA sequences. The karyotype of the thymallid fish is thought to experienced numerous pericentric inversions and internal telomeric sites (ITSs) observed at the pericentromeric regions of the six European grayling metacentric chromosomes are likely relics of the these rearrangements. None of the ITS sites matched either chromosome 21 or NOR bearing chromosomes.
dc.description.sponsorshipUniversity of Warmia and Mazury in Olsztyn, Poland (0804.0809)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1007/s10709-013-9740-7
dc.identifier.issn0016-6707
dc.identifier.issn1573-6857
dc.identifier.urihttp://hdl.handle.net/10400.1/11223
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectThymallus thymallus
dc.subjectTransposable elements
dc.subjectOrganizer regions
dc.subjectEvolution
dc.subjectPisces
dc.subjectFish
dc.subjectSalmonidae
dc.subjectHeterochromatin
dc.subjectLocalization
dc.subjectPolymorphism
dc.titlePericentromeric location of the telomeric DNA sequences on the European grayling chromosomes
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage416
oaire.citation.issue10-dez
oaire.citation.startPage409
oaire.citation.titleGenetica
oaire.citation.volume141
rcaap.rightsopenAccess
rcaap.typearticle

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