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Insights on the anti-inflammatory and antitumor activities of extracts from the marine green alga Codium decorticatum

2020-08Journal article Restricted access
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dc.contributor.authorZbakh, Hanaa
dc.contributor.authorSalhi, Ghizlane
dc.contributor.authorBochkov, Valery
dc.contributor.authorCiudad, Carles J.
dc.contributor.authorNoe, Veronique
dc.contributor.authorHassoun, Mustapha
dc.contributor.authorRiadi, Hassane
dc.date.accessioned2021-06-24T11:35:23Z
dc.date.available2021-06-24T11:35:23Z
dc.date.issued2020-08
dc.description.abstractIntroduction: The Codium genus has been shown to be an important source of marine bioactive compounds for pharmaceutical and nutraceutical applications. The present study was conducted to investigate the anti-inflammatory activity of the n-hexane (Extr 1), dichloromethane (Extr 2) and acetone/methanol (Extr 3) extracts of the green alga Codium decorticatum, as well as their antitumor effects on several cancer cell lines. Methods: In order to evaluate the anti-inflammatory effect of the extracts of C. decorticatum on the production of the pro-inflammatory proteins, we have undertaken analyses of LPSand TNF-alpha-stimulated endothelial cells treated with extracts using enzyme-linked immunosorbent assay (ELISA). Real-time PCR (qPCR) was used to modulate the mRNA expression of inflammatory genes. For the antitumor activity, in vitro cytotoxicity was determined by MTT assay and apoptosis was determined by flow cytometry. Results: Our results revealed that the Extr 2 and Extr 3 dramatically inhibited the expression of the pro -inflammatory cytokine Interleukin-8 (IL-8) in LPSand TNF-alpha-stimulated endothelial cells. We also demonstrated that Extr 2 suppressed the LPS-induced mRNA expression of E-selectin and IL-8. Furthermore, Extr 2 showed cytotoxic activity against the HeLa cell line (IC50 = 37.22 mu g/mL), by inducing apoptosis at highest concentrations. Conclusions: The present data suggest that the seaweed C. decorticatum has the anti-inflammatory properties in endothelial cells, together with the anticancer effect on cervix cancer cells, which may have a potential therapeutic use for inflammatory diseases and cancer. Although, its application as nutraceuticals and functional food could be also considered.
dc.description.sponsorshipL'Oreal-UNESCO for Women in Science Maghreb Fellowships, 2017/2018
dc.description.sponsorshipEU-METALIC II Erasmus Mundus project
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.eujim.2020.101170
dc.identifier.issn1876-3820
dc.identifier.urihttp://hdl.handle.net/10400.1/16422
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectCodium decorticatum
dc.subjectAnti-inflammatory
dc.subjectPro-inflammatory cytokines
dc.subjectAntitumor
dc.subjectApoptosis
dc.subjectMarine green alga
dc.subject.otherIntegrative & Complementary Medicine
dc.titleInsights on the anti-inflammatory and antitumor activities of extracts from the marine green alga Codium decorticatum
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage101170
oaire.citation.titleEuropean Journal of Integrative Medicine
oaire.citation.volume37
person.familyNameZbakh
person.givenNameHanaa
person.identifier.orcid0000-0001-6131-7827
person.identifier.scopus-author-id54912131600
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication28b3f2ee-340b-4813-bce2-db916b340550
relation.isAuthorOfPublication.latestForDiscovery28b3f2ee-340b-4813-bce2-db916b340550

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