dc.contributor.author	Pinto, Patricia IS
dc.contributor.author	Matsumura, H.
dc.contributor.author	Thorne, Michael A. S.
dc.contributor.author	Power, Deborah
dc.contributor.author	Terauchi, Ryohei
dc.contributor.author	Reinhardt, Richard
dc.contributor.author	Canario, Adelino V. M.
dc.date.accessioned	2012-02-14T22:26:08Z
dc.date.available	2012-02-14T22:26:08Z
dc.date.issued	2010-08-17
dc.date.updated	2012-02-06T16:10:38Z
dc.description.abstract	Abstract Background Calcium ion is tightly regulated in body fluids and for euryhaline fish, which are exposed to rapid changes in environmental [Ca2+], homeostasis is especially challenging. The gill is the main organ of active calcium uptake and therefore plays a crucial role in the maintenance of calcium ion homeostasis. To study the molecular basis of the short-term responses to changing calcium availability, the whole gill transcriptome obtained by Super Serial Analysis of Gene Expression (SuperSAGE) of the euryhaline teleost green spotted puffer fish, Tetraodon nigroviridis, exposed to water with altered [Ca2+] was analysed. Results Transfer of T. nigroviridis from 10 ppt water salinity containing 2.9 mM Ca2+ to high (10 mM Ca2+ ) and low (0.01 mM Ca2+) calcium water of similar salinity for 2-12 h resulted in 1,339 differentially expressed SuperSAGE tags (26-bp transcript identifiers) in gills. Of these 869 tags (65%) were mapped to T. nigroviridis cDNAs or genomic DNA and 497 (57%) were assigned to known proteins. Thirteen percent of the genes matched multiple tags indicating alternative RNA transcripts. The main enriched gene ontology groups belong to Ca2+ signaling/homeostasis but also muscle contraction, cytoskeleton, energy production/homeostasis and tissue remodeling. K-means clustering identified co-expressed transcripts with distinct patterns in response to water [Ca2+] and exposure time. Conclusions The generated transcript expression patterns provide a framework of novel water calcium-responsive genes in the gill during the initial response after transfer to different [Ca2+]. This molecular response entails initial perception of alterations, activation of signaling networks and effectors and suggests active remodeling of cytoskeletal proteins during the initial acclimation process. Genes related to energy production and energy homeostasis are also up-regulated, probably reflecting the increased energetic needs of the acclimation response. This study is the first genome-wide transcriptome analysis of fish gills and is an important resource for future research on the short-term mechanisms involved in the gill acclimation responses to environmental Ca2+ changes and osmoregulation.	por
dc.description.version	Peer Reviewed
dc.identifier.citation	BMC Genomics. 2010 Aug 17;11(1):476	por
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2164-11-476
dc.identifier.other	AUT: DPO00386; ACA00258;
dc.identifier.uri	http://hdl.handle.net/10400.1/899
dc.language.iso	eng	por
dc.language.rfc3066	eng
dc.peerreviewed	yes	por
dc.rights.holder	Pinto et al.; licensee BioMed Central Ltd.
dc.title	Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish	por
dc.type	journal article
dspace.entity.type	Publication
person.familyName	Pinto
person.familyName	Power
person.familyName	Canario
person.givenName	Patricia IS
person.givenName	Deborah Mary
person.givenName	Adelino
person.identifier	643457
person.identifier	143624
person.identifier.ciencia-id	E51D-5CCB-B1C6
person.identifier.ciencia-id	891A-8A44-3CAE
person.identifier.ciencia-id	1F1E-D3B3-F804
person.identifier.orcid	0000-0001-7854-3898
person.identifier.orcid	0000-0003-1366-0246
person.identifier.orcid	0000-0002-6244-6468
person.identifier.rid	M-3817-2013
person.identifier.rid	C-7942-2009
person.identifier.scopus-author-id	10240774300
person.identifier.scopus-author-id	7101806760
person.identifier.scopus-author-id	56568523700
rcaap.rights	openAccess	por
rcaap.type	article	por
relation.isAuthorOfPublication	64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication	c68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication	5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery	64bc526e-5281-44e5-91eb-5743436109f9

Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish

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