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Evolutionary conservation of TFIIH subunits: Implications for the use of zebrafish as a model to study TFIIH function and regulation

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dc.contributor.authorSilva, IAL
dc.contributor.authorCox, C. J.
dc.contributor.authorLeite, Ricardo
dc.contributor.authorCancela, Leonor
dc.contributor.authorConceição, N.
dc.date.accessioned2014-10-27T14:20:11Z
dc.date.available2014-10-27T14:20:11Z
dc.date.issued2014
dc.date.updated2014-10-24T09:48:09Z
dc.description.abstractTranscriptional factor IIH (TFIIH) is involved in cell cycle regulation, nucleotide excision repair, and gene transcription. Mutations in three of its subunits, XPB, XPD, and TTDA, lead to human recessive genetic disorders such as trichothiodystrophy and xeroderma pigmentosum, the latter of which is sometimes associated with Cockayne's syndrome. In the present study, we investigate the sequence conservation of TFIIH subunits among several teleost fish species and compare their characteristics and putative regulation by transcription factors to those of human and zebrafish. We report the following findings: (i) comparisons among protein sequences revealed a high sequence identity for each TFIIH subunit analysed; (ii) among transcription factors identified as putative regulators, OCT1 and AP1 have the highest binding-site frequencies in the promoters of TFIIH genes, and (iii) TFIIH genes have alternatively spliced isoforms. Finally, we compared the protein primary structure in human and zebrafish of XPD and XPB – twoimportant ATP-dependent helicases that catalyse the unwinding of the DNA duplex at promoters during transcription – highlighting the conservation of domain regions such as the helicase domains. Our study suggests that zebrafish, a widely used model for many human diseases, could also act as an important model to study the function of TFIIH complex in repair and transcription regulation in humans.por
dc.description.sponsorshipThis research was partially supported by the European Regional Development Fund (ERDF) through the COMPETE-Operational Competitiveness Programme and national funds through FCT — Foundation for Science and Technology, under the project “PEst-C/MAR/LA0015/2011”.por
dc.identifier.citationSilva, I.A.L.; Cox, C.J.; Leite, R.B.; Cancela, M.L.; Conceição, N. Evolutionary conservation of TFIIH subunits: Implications for the use of zebrafish as a model to study TFIIH function and regulation, Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, 172-1, 1, 9-20, 2014.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.cbpb.2014.03.007
dc.identifier.issn1096-4959
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/5526
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectTranscriptional factor IIH (TFIIH)por
dc.subjectXeroderma pigmentosum D (XPD)por
dc.subjectXeroderma pigmentosum B (XPB)por
dc.subjectZebrafishpor
dc.subjectTranscription regulationpor
dc.titleEvolutionary conservation of TFIIH subunits: Implications for the use of zebrafish as a model to study TFIIH function and regulationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage20por
oaire.citation.issue1por
oaire.citation.startPage9por
oaire.citation.titleComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biologypor
oaire.citation.volume172-1por
person.familyNameSilva
person.familyNameCox
person.familyNameLeite
person.familyNameCancela
person.givenNameIris
person.givenNameCymon
person.givenNameRicardo
person.givenNameM. Leonor
person.identifier447622
person.identifier.ciencia-id8319-EDFC-1260
person.identifier.ciencia-id6B15-9771-1D04
person.identifier.ciencia-idF115-256E-C84D
person.identifier.orcid0000-0002-8062-5558
person.identifier.orcid0000-0002-4927-979X
person.identifier.orcid0000-0002-9622-3895
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridU-5740-2017
person.identifier.ridD-1303-2012
person.identifier.ridB-3389-2008
person.identifier.scopus-author-id57196094239
person.identifier.scopus-author-id7402112716
person.identifier.scopus-author-id8362650900
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery3fb360e0-a481-486e-96ca-af9f6ecd6eb8

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