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Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae

2003-01Journal article Restricted access
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dc.contributor.authorAlbers, E.
dc.contributor.authorLaizé, Vincent
dc.contributor.authorBlomberg, A.
dc.contributor.authorHohmann, S.
dc.contributor.authorGustafsson, L.
dc.date.accessioned2014-06-13T14:33:22Z
dc.date.available2014-06-13T14:33:22Z
dc.date.issued2003-01
dc.date.updated2014-06-03T10:24:05Z
dc.descriptionTexto integral disponível através do editorpor
dc.description.abstractTwo genes YER081W and YIL074C, renamed SER3 and SER33, respectively, which encode phosphoglycerate dehydrogenases in Saccharomyces cerevisiae were identified. These dehydrogenases catalyze the first reaction of serine and glycine biosynthesis from the glycolytic metabolite 3-phosphoglycerate. Unlike either single mutant, the ser3Delta ser33Delta double mutant lacks detectable phosphoglycerate dehydrogenase activity and is auxotrophic for serine or glycine for growth on glucose media. However, the requirement for the SER-dependent "phosphoglycerate pathway" is conditional since the "glyoxylate" route of serine/glycine biosynthesis is glucose-repressed. Thus, in cells grown on ethanol both expression and activity of all SER-encoded proteins are low, including the remaining enzymes of the phosphoglycerate pathway, Ser1p and Ser2p. Moreover the available nitrogen source regulates the expression of SER genes. However, for only SER33, and not SER3, expression was regulated in relation to the available nitrogen source in a coordinated fashion with SER1 and SER2. Based on these mRNA data together with data on enzyme activities, Ser33p is likely to be the main isoenzyme of the phosphoglycerate pathway during growth on glucose. Moreover, since phosphoglycerate dehydrogenase activity requires NAD(+) as cofactor, deletion of SER3 and SER33 markedly affected redox metabolism as shown by substrate and product analysis.por
dc.identifier.citationAlbers E; Laizé V; Blomberg A; Hohmann S; Gustafsson L. Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae, Journal of Biological Chemistry, 278, 12, 10264-10272, 2003.por
dc.identifier.doihttp://dx.doi.org/ 10.1074/jbc.M211692200
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10400.1/4306
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Society for Biochemistry and Molecular Biologypor
dc.relation.publisherversionhttp://www.jbc.org/content/278/12/10264.longpor
dc.subjectPhosphoglyceratepor
dc.subjectDehydrogenasespor
dc.titleSer3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiaepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage10272por
oaire.citation.startPage10264por
oaire.citation.titleJournal of Biological Chemistrypor
oaire.citation.volume278por
person.familyNameLaizé
person.givenNameVincent
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscoveryb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f

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