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Copper Complexes with 1,10-Phenanthroline Derivatives: Underlying Factors Affecting Their Cytotoxicity

2020-07Journal article Open Access
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dc.contributor.authorNunes, Patrique
dc.contributor.authorCorreia, Isabel
dc.contributor.authorMarques, Fernanda
dc.contributor.authorMatos, Antonio Pedro
dc.contributor.authordos Santos, Margarida M. C.
dc.contributor.authorAzevedo, Cristina G.
dc.contributor.authorCapelo, Jose-Luis
dc.contributor.authorSantos, Hugo M.
dc.contributor.authorGama, Sofia
dc.contributor.authorPinheiro, Teresa
dc.contributor.authorCavaco, Isabel
dc.contributor.authorPessoa, Joao Costa
dc.date.accessioned2021-06-24T11:35:26Z
dc.date.available2021-06-24T11:35:26Z
dc.date.issued2020-07
dc.description.abstractThe interpretation of in vitro cytotoxicity data of Cu(II)-1,10-phenanthroline (phen) complexes normally does not take into account the speciation that complexes undergo in cell incubation media and its implications in cellular uptake and mechanisms of action. We synthesize and test the activity of several distinct Cu(II)-phen compounds; up to 24 h of incubation, the cytotoxic activity differs for the Cu complexes and the corresponding free ligands, but for longer incubation times (e.g., 72 h), all compounds display similar activity. Combining the use of several spectroscopic, spectrometric, and electrochemical techniques, the speciation of Cu-phen compounds in cell incubation media is evaluated, indicating that the originally added complex almost totally decomposed and that Cu(II) and phen are mainly bound to bovine serum albumin. Several methods are used to disclose relationships between structure, activity, speciation in incubation media, cellular uptake, distribution of Cu in cells, and cytotoxicity. Contrary to what is reported in most studies, we conclude that interaction with cell components and cell death involves the separate action of Cu ions and phen molecules, not [Cu(phen)(n)] species. This conclusion should similarly apply to many other Cu-ligand systems reported to date.
dc.description.sponsorshipFundacao para a Ciencia e Tecnologia (FCT)Portuguese Foundation for Science and TechnologyEuropean Commission [UID/Multi/04349/2019, UIDB/00100/2020, UID/QUI/50006/2019, UID/BIO/04565/2020]
dc.description.sponsorshipFCTPortuguese Foundation for Science and TechnologyEuropean Commission [IF/00841/2012, IF/00007/2015, SFRH/BD/108743/2015]
dc.description.sponsorshipPrograma Operacional Regional de Lisboa 2020 [007317]
dc.description.sponsorshipPROTEOMASS Scientific Society
dc.description.sponsorshipCOST Action, NECTAR (Network for Equilibria and Chemical Thermodynamics Advanced Research) - COST (European Cooperation in Science and Technology) [CA18202]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1021/acs.inorgchem.0c00925
dc.identifier.issn0020-1669
dc.identifier.urihttp://hdl.handle.net/10400.1/16439
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Chemical Society
dc.relationInteraction of biomacromolecules with ionic liquids
dc.relationTherapeutic transition-metal complexes of vitamin B6 related compounds
dc.relationCentre for Nuclear Sciences and Technologies
dc.relationCentro de Química Estrutural
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.subject.otherChemistry
dc.titleCopper Complexes with 1,10-Phenanthroline Derivatives: Underlying Factors Affecting Their Cytotoxicity
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInteraction of biomacromolecules with ionic liquids
oaire.awardTitleTherapeutic transition-metal complexes of vitamin B6 related compounds
oaire.awardTitleCentre for Nuclear Sciences and Technologies
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00841%2F2012%2FCP0171%2FCT0009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00007%2F2015%2FCP1293%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F108743%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04349%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F50006%2F2019/PT
oaire.citation.endPage9134
oaire.citation.issue13
oaire.citation.startPage9116
oaire.citation.titleInorganic Chemistry
oaire.citation.volume59
oaire.fundingStreamInvestigador FCT
oaire.fundingStreamInvestigador FCT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePALMA ANTUNES CAVACO
person.givenNameISABEL MARIA
person.identifierH-9087-2012
person.identifier.ciencia-idD515-FB5E-1205
person.identifier.orcid0000-0001-8651-9054
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
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