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Developmental ontogeny of prolactin and prolactin receptor in the sea bream (Sparus aurata)

2004Journal article Restricted access
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dc.contributor.authorSantos, C. R. A.
dc.contributor.authorCavaco, J. E. B.
dc.contributor.authorIngleton, P. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-21T16:03:59Z
dc.date.available2014-10-21T16:03:59Z
dc.date.issued2004
dc.description.abstractThe expression of PRL and its receptor (PRLR) were characterised during sea bream embryonic and larval development, by semi-quantitative and quantitative RT-PCR, respectively, until 46 days post-hatch (DPH). Immunocytochemistry with antisera specific for sea bream PRLR was carried out with larval sections from hatching up to 46 DPH. A single transcript of PRL (1.35 Kb) and PRLR (2.8 Kb) identical to the transcripts previously characterised in adult tissue, are present in sea bream embryos and larvae. PRL expression is first detectable at neurula and in all samples collected thereafter. The lowest levels of PRL mRNA are detected in sea bream embryos up until neurula when expression starts to increase. The maximal levels of PRL expression were detected at 24 DPH. PRLR transcripts first appear at 12 h post-fertilisation (0:002qmol=lg total larvae RNA) (blastula) and increase significantly during gastrulation (0:245qmol=lg total larvae RNA) reaching a maximum at 2 DPH (0:281qmol=lg total larvae RNA). After hatching a significant reduction in PRLR expression is observed which reaches a minimum at 4 DPH (0:103qmol=lg total larvae RNA), gradually increasing thereafter. Immunocytochemistry revealed the presence of PRLR in early post-hatching stages of larvae in tissues derived from all three germ layers.por
dc.description.sponsorshipWe thank Dr. Juan Fuentes for assistance with the statistical analysis, Dr. Juan Miguel Mancera and Cultivos Pisc ıcolas Marinos (Cupimar, San Fernando, C adiz, Spain) for providing sea bream eggs and larvae and Ms Liliana Anjos for technical assistance. C.R.A. Santos and J.E.B. Cavaco are in receipt of FCT postdoctoral grants (PRAXIS XXI/BPD/22040/99 and SFRH/BPD 5555). This work was supported by a FCT grant (PRAXIS XXI- 2/2.1/BIA/211/94) and FCT ‘‘Financiamento Plurianual’’.por
dc.identifier.citationC.R.A. Santos, J.E.B. Cavaco, P.M. Ingleton, and D.M. Power. "Developmental ontogeny of prolactin and prolactin receptor in the sea bream (Sparus aurata)" in General and Comparative Endocrinology 132 (2003) 304–314.por
dc.identifier.doihttp://dx.doi.org/10.1016/S0016-6480(03)00083-2
dc.identifier.doi0016-6480
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5396
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.titleDevelopmental ontogeny of prolactin and prolactin receptor in the sea bream (Sparus aurata)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage314por
oaire.citation.startPage304por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume132por
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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