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Effects of calcium carbonate inclusion in low fishmeal diets on growth, gastrointestinal pH, digestive enzyme activity and gut bacterial community of European sea bass (Dicentrarchus labrax L.) juveniles

2019-08Journal article Open access
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dc.contributor.authorParma, Luca
dc.contributor.authorYufera, Manuel
dc.contributor.authorNavarro-Guillen, Carmen
dc.contributor.authorMoyano, Francisco J.
dc.contributor.authorSoverini, Matteo
dc.contributor.authorD'Amico, Federica
dc.contributor.authorCandela, Marco
dc.contributor.authorFontanillas, Ramon
dc.contributor.authorGatta, Pier Paolo
dc.contributor.authorBonaldo, Alessio
dc.date.accessioned2020-07-24T10:51:47Z
dc.date.available2020-07-24T10:51:47Z
dc.date.issued2019-08
dc.description.abstractFishmeal (FM) possesses one of the highest buffering capacities in comparison to most alternative vegetable aquafeed ingredients and its decreasing content in current formulations might affect the ideal gastrointestinal environment for digestive enzyme action and gut bacterial community of carnivorous fish species. A study was undertaken, therefore, to assess growth response, gastrointestinal pH, digestive enzyme activity and gut bacterial community of European sea bass (Dicentrarchus labrax L.) juveniles fed low FM diets (10% FM) with or without a feed buffering additive, calcium carbonate (FM10 + B and FM10, respectively) in comparison to a standard FM diet (20% FM, FM20). Three isonitrogenous and isolipidic extruded diets were fed to triplicate fish groups of 80 individuals (initial weight: 23 g) to overfeeding over 64 days. No significant differences due to low FM dietary levels were observed in final body weight, specific growth rate, feed intake, feed and protein efficiency. Low FM diet did not affect gastrointestinal pH in the stomach, anterior intestine, mid-intestine and hindgut at 0, 4, 8 and 12 hours post meal (hpm) while the inclusion of calcium carbonate in low FM diet seems to slightly increase the pH in the hindgut at 12 hpm. The absence of significant differences of pepsin, chymotrypsin, amylase and lipase activity suggests a comparable digestive efficiency among treatments although trypsin activity was slightly reduced in low FM diets after 4 hpm. Decreasing FM content seems to exert an effect on the overall gut bacterial community analysed by next-generation sequencing even if no significant effects on specific bacterial component were detected. The gut bacterial community in all the treatments was particularly rich in lactic acid bacteria such as Lactobacillus spp. which may provide important beneficial functions for the host and be associated with a healthy intestinal epithelium. According to the results, increasing the feed buffering capacity does not seem to improve digestive conditions while it is feasible to include 10% FM dietary level in practical formulation for European sea bass juveniles without negatively affecting growth, feed efficiency and digestive luminal conditions.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.aquaculture.2019.05.064
dc.identifier.issn0044-8486
dc.identifier.issn1873-5622
dc.identifier.urihttp://hdl.handle.net/10400.1/14270
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectBream Sparus-Aurata
dc.subjectSoybean-meal
dc.subjectProduction performance
dc.subjectVegetable meals
dc.subjectDaily rhythms
dc.subjectReplacement
dc.subjectProtein
dc.subjectOil
dc.subjectCapacity
dc.subjectTeleost
dc.subjectEuropean sea bass
dc.subjectFishmeal replacement
dc.subjectFeed buffering capacity
dc.subjectGastrointestinal pH
dc.subjectDigestive enzyme
dc.subjectGut bacterial community
dc.titleEffects of calcium carbonate inclusion in low fishmeal diets on growth, gastrointestinal pH, digestive enzyme activity and gut bacterial community of European sea bass (Dicentrarchus labrax L.) juveniles
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage292
oaire.citation.startPage283
oaire.citation.titleAquaculture
oaire.citation.volume510
person.familyNameParma
person.givenNameLuca
person.identifier.orcid0000-0003-4115-4383
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication666e717f-4eb2-4828-aad1-dc4318d4f9ab
relation.isAuthorOfPublication.latestForDiscovery666e717f-4eb2-4828-aad1-dc4318d4f9ab

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